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Titolo:
SECRETED PROTEOPHOSPHOGLYCAN OF LEISHMANIA-MEXICANA AMASTIGOTES ACTIVATES COMPLEMENT BY TRIGGERING THE MANNAN-BINDING LECTIN PATHWAY
Autore:
PETERS C; KAWAKAMI M; KAUL M; ILG T; OVERATH P; AEBISCHER T;
Indirizzi:
MAX PLANCK INST BIOL,ABT MEMBRANBIOCHEM,CORRENSSTR 38 D-72076 TUBINGEN GERMANY MAX PLANCK INST BIOL,ABT MEMBRANBIOCHEM D-72076 TUBINGEN GERMANY KITASATO INST,BASIC RES CTR TOKYO 108 JAPAN UNIV MAINZ,INST MED MIKROBIOL & HYG D-6500 MAINZ GERMANY
Titolo Testata:
European Journal of Immunology
fascicolo: 10, volume: 27, anno: 1997,
pagine: 2666 - 2672
SICI:
0014-2980(1997)27:10<2666:SPOLAA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
RA-REACTIVE FACTOR; ACID-PHOSPHATASE; HUMAN-SERUM; BACTERICIDAL FACTOR; MAJOR AMASTIGOTES; SERINE-PROTEASE; MEDIATED LYSIS; PROMASTIGOTES; MACROPHAGES; SURFACE;
Keywords:
MANNAN BINDING PROTEIN; GLYCOCONJUGATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
C. Peters et al., "SECRETED PROTEOPHOSPHOGLYCAN OF LEISHMANIA-MEXICANA AMASTIGOTES ACTIVATES COMPLEMENT BY TRIGGERING THE MANNAN-BINDING LECTIN PATHWAY", European Journal of Immunology, 27(10), 1997, pp. 2666-2672

Abstract

Cutaneous lesions induced by infection of mice with the protozoan parasite, Leishmania mexicana contain abundant amounts of a high molecular mass proteophosphoglycan (PPG), which is secreted by the amastigote stage residing in phagolysosomes of macrophages and can then be released into the tissue upon rupture of the infected cells. Amastigote PPG forms sausage-shaped but soluble particles and belongs to a novel class of serine-rich proteins that are extensively O-glycosylated by phosphooligosaccharides capped by mannooligosaccharides. The purified molecule is shown here to efficiently activate complement (C) and deplete hemolytic activity of normal serum and may prevent the opsonization of L. mexicana amastigotes. Complement activation is Ca2+ dependent but does not depend on antibodies or the complement component C1. PPG bindsto serum mannan binding protein (MBP), thus activating the MBP-associated serine protease, P100. Subsequently, the C cascade is triggered through C3 leading to covalent modification probably of carbohydrate hydroxyls of PPG by C3 fragments. Thus, PPG is able to activate C via the mannan binding lectin pathway which is unusual for secreted, solubleproducts of microbial origin. The proteophosphoglycan-induced complement activation is postulated to contribute to the lesion development and pathology caused by the parasite.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 13:35:52