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Titolo:
SELECTIVE INTRAPORTAL TRANSPLANTATION OF DII-MARKED ISOLATED RAT HEPATOCYTES
Autore:
MARKUS PM; KOENIG S; KRAUSE P; BECKER H;
Indirizzi:
UNIV GOTTINGEN,DEPT GEN SURG,ROBERT KOCHSTR 40 D-37075 GOTTINGEN GERMANY
Titolo Testata:
Cell transplantation
fascicolo: 5, volume: 6, anno: 1997,
pagine: 455 - 462
SICI:
0963-6897(1997)6:5<455:SITODI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
MICROCARRIER-ATTACHED HEPATOCYTES; ACUTE LIVER-FAILURE; HEPATOCELLULAR TRANSPLANTATION; HEPATIC-FAILURE; GUNN-RATS; CELLS; ENGRAFTMENT; SURVIVAL; SPLEEN;
Keywords:
INTRAPORTAL TRANSPLANTATION; DII-MARKED ISOLATED RAT HEPATOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
P.M. Markus et al., "SELECTIVE INTRAPORTAL TRANSPLANTATION OF DII-MARKED ISOLATED RAT HEPATOCYTES", Cell transplantation, 6(5), 1997, pp. 455-462

Abstract

Transplantation of isolated hepatocytes is a promising alternative toorthotopic liver transplantation in experimental animal models with acute hepatic failure and hereditary enzyme defects, Conventional lightmicroscopy identification of hepatocytes within recipient livers has been limited due to the inability to distinguish between donor and recipient liver cells, In this study, we labeled hepatocytes intracellularly with the fluorescent dye DiI-18 prior to selective intraportal or intrasplenic transplantation. Syngeneic LEW rat hepatocytes were isolated and 2 x 10(7) fluorescence-labeled cells were transplanted by intraportal infusion selectively into 2/3 of the recipient liver lobules to avoid lethal portal hypertension, Rats were sacrificed on postop days 1, 3, 5, 10, 20, and 40, Histological examination was performed using light and fluorescence microscopy counterstained by light green dye,The quantity of transplanted hepatocytes residing within the recipient liver was determined by FAGS analysis after enzymatic digestion of the recipient liver lobules. Engrafted hepatocytes were identified in the periportal regions of transplanted liver lobules, The stained hepatocytes were retrieved up to 20 days postop using fluorescent microscopy, Using FAGS analysis the number of labeled hepatocytes was found to diminish over time following transplantation from 2.1% on postop day 1to 0.5% on day 10, Labeled hepatocytes transplanted into the spleen were retrieved in clusters up to 20 days postop (the last day of observation), Furthermore, the migration of labeled hepatocytes from spleen to liver parenchyma was observed following intrasplenic transplantation, However, after selective intraportal transplantation, only fluorescent debris was found in splenic and pulmonary tissue upon examination of various organs, This article describes the method of fluorescent labeling of rat hepatocytes and reports the feasibility and limitations of using DiI-18 as a marker, (C) 1997 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/21 alle ore 15:08:59