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Titolo:
DIFFERENTIAL STIMULATION BY CCAAT ENHANCER-BINDING PROTEIN ALPHA-ISOFORM OF THE ESTROGEN-ACTIVATED PROMOTER OF THE VERY-LOW-DENSITY APOLIPOPROTEIN-II GENE/
Autore:
CALKHOVEN CF; SNIPPE L; AB G;
Indirizzi:
UNIV GRONINGEN,BIOCHEM LAB,NIJENBORGH 4 NL-9747 AG GRONINGEN NETHERLANDS UNIV GRONINGEN,BIOCHEM LAB NL-9747 AG GRONINGEN NETHERLANDS
Titolo Testata:
European journal of biochemistry
fascicolo: 1, volume: 249, anno: 1997,
pagine: 113 - 120
SICI:
0014-2956(1997)249:1<113:DSBCEP>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
C/EBP-RELATED PROTEINS; ACUTE-PHASE RESPONSE; TRANSCRIPTION-FACTOR; MESSENGER-RNA; ALBUMIN PROMOTER; NUCLEAR FACTOR; ADIPOCYTE DIFFERENTIATION; GLUCOCORTICOID INDUCTION; NF-M; EXPRESSION;
Keywords:
CCAAT/ENHANCER-BINDING PROTEIN ALPHA; ESTROGEN RECEPTOR; VERY-LOW-DENSITY APOLIPOPROTEIN II; ISOFORM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
C.F. Calkhoven et al., "DIFFERENTIAL STIMULATION BY CCAAT ENHANCER-BINDING PROTEIN ALPHA-ISOFORM OF THE ESTROGEN-ACTIVATED PROMOTER OF THE VERY-LOW-DENSITY APOLIPOPROTEIN-II GENE/", European journal of biochemistry, 249(1), 1997, pp. 113-120

Abstract

The transcription factors CCAAT/enhancer-binding proteins alpha and beta (C/EBP alpha and C/EBP beta) are highly expressed in liver and arebelieved to function in maintaining the differentiated state of the hepatocytes, C/EBP alpha appears to be a critical regulator of genes involved in metabolic processes. We are interested in the roles of C/EBPin the expression of the very-low-density apolipoprotein II (apoVLDL II) gene. This gene encodes an avian yolk protein, is induced by estrogens and is only expressed in liver. To examine the role of C/EBP in apoVLDL II expression, footprinting and electromobility-shift analysis were performed. For three of the protein-binding sites in the apoVLDL II promoter region, C/EBP alpha and C/EBP beta were identified as the major DNA-binding activities. For one of the C/EBP genes, C/EBP alpha,the effect of the gene products on apoVLDL II transcription was examined. From transfection experiments we conclude that maximal estrogen-dependent activity of the apoVLDL II promoter requires the dual action of the estrogen receptor and C/EBP. The level of activity is differentdepending on the nature of the C/EBP alpha translational isoform transfected, the full-length C/EBP alpha polypeptide being the most activeisoform and the N-terminally truncated isoform being moderately active. The present results suggest a role of C/EBP alpha translational isoform ratio in the modulation of expression of C/EBP target genes, suchas those involved in metabolic processes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 08:58:52