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Titolo: INDUCTION OF CYCLOOXYGENASE-2 IN HUMAN SAPHENOUS-VEIN AND INTERNAL MAMMARY ARTERY
Autore: BISHOPBAILEY D; PEPPER JR; HADDAD EB; NEWTON R; LARKIN SW; MITCHELL JA;
- Indirizzi:
- ROYAL BROMPTON NATL HEART & LUNG HOSP,DEPT ANAESTHET & CRIT CARE MED,SYDNEY ST LONDON SW3 6NP ENGLAND ROYAL BROMPTON NATL HEART & LUNG HOSP,DEPT ANAESTHET & CRIT CARE MED LONDON SW3 6NP ENGLAND UNIV LONDON SCH PHARM,NATL HEART & LUNG INST,DEPT APPL PHARMACOL LONDON ENGLAND UNIV LONDON SCH PHARM,NATL HEART & LUNG INST,DEPT THORAC MED LONDON ENGLAND ROYAL BROMPTON NATL HEART & LUNG HOSP,DEPT CARDIOTHORAC SURG LONDON SW3 6NP ENGLAND
- Titolo Testata:
- Arteriosclerosis, thrombosis, and vascular biology
fascicolo: 9,
volume: 17,
anno: 1997,
pagine: 1644 - 1648
- SICI:
- 1079-5642(1997)17:9<1644:IOCIHS>2.0.ZU;2-R
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- SMOOTH-MUSCLE CELLS; NITRIC-OXIDE SYNTHASE; ENDOTHELIAL-CELLS; ORGAN-CULTURE; RABBIT AORTA; IN-VIVO; PROSTAGLANDIN; GROWTH; ATHEROSCLEROSIS; INTERLEUKIN-1;
- Keywords:
- CYCLOOXYGENASE; SAPHENOUS VEIN; INTERNAL MAMMARY ARTERY; PROSTACYCLIN;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Science Citation Index Expanded
- Citazioni:
- 35
- Recensione:
- Indirizzi per estratti:
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- Citazione:
- D. Bishopbailey et al., "INDUCTION OF CYCLOOXYGENASE-2 IN HUMAN SAPHENOUS-VEIN AND INTERNAL MAMMARY ARTERY", Arteriosclerosis, thrombosis, and vascular biology, 17(9), 1997, pp. 1644-1648
Abstract
Within vessels, cyclooxygenase (COX) is expressed constitutively (COX-1) in endothelial cells where its production of prostacyclin is thought to contribute to the maintenance of vascular integrity. Recently, anovel isoform of COX, COX-2, has been described that is induced in animal arterial vessels after physical damage or exposure to proinflammatory cytokines. However, induction of COX-2 in human vessels has not been characterized. Moreover, the relative ability of arteries and veins to express COX-2 has not been addressed. Thus, we have compared the ability of segments of human saphenous vein and internal mammary artery, obtained from the same patient, to express COX-2 activity and mRNA after organ culture in the presence and absence of interleukin-1 beta. COX-2 metabolites, measured by radioimmunoassay, were released by both the internal mammary artery and saphenous vein in the following rankorder: prostaglandin E-2 greater than or equal to prostacyclin thromboxane A(2). Inclusion of interleukin-1 beta in the culture medium increased the release of prostanoids by the saphenous vein but not by the internal mammary artery. However, the selective COX-2 inhibitor NS-398significantly attenuated prostacyclin release from both tissues. Northern blot analysis showed no detectable COX-2 mRNA in freshly preparedsaphenous vein or internal mammary artery. In contrast, after 48 hours in organ culture, low levels of COX-2 mRNA were detected in both internal mammary artery and saphenous vein, an effect that was greatly increased by interleukin-1 beta. These observations show that COX-2 is induced in human saphenous vein and internal mammary artery and suggestthat this may occur in humans after coronary artery bypass graft surgery. The induction of COX-2 and subsequent release of prostacyclin mayrepresent an endogenous defense mechanism against endothelial damage incurred during surgical preparation of these vessels for bypass.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 01:33:21