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Titolo:
IDENTIFICATION OF NICOTINIC ACETYLCHOLINE-RECEPTORS ON LYMPHOCYTES INTHE PERIPHERY AS WELL AS THYMUS IN MICE
Autore:
TOYABE S; IIAI T; FUKUDA M; KAWAMURA T; SUZUKI S; UCHIYAMA M; ABO T;
Indirizzi:
NIIGATA UNIV,SCH MED,DEPT IMMUNOL,ASAHIMACHI 1 NIIGATA 951 JAPAN NIIGATA UNIV,SCH MED,DEPT IMMUNOL NIIGATA 951 JAPAN NIIGATA UNIV,SCH MED,DEPT PAEDIAT NIIGATA 951 JAPAN SAKAMACHI HOSP NIIGATA JAPAN
Titolo Testata:
Immunology
fascicolo: 2, volume: 92, anno: 1997,
pagine: 201 - 205
SICI:
0019-2805(1997)92:2<201:IONAOL>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYASTHENIA-GRAVIS; GENE-EXPRESSION; MESSENGER-RNAS; ALPHA-SUBUNIT; CELLS; THYMOMAS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
S. Toyabe et al., "IDENTIFICATION OF NICOTINIC ACETYLCHOLINE-RECEPTORS ON LYMPHOCYTES INTHE PERIPHERY AS WELL AS THYMUS IN MICE", Immunology, 92(2), 1997, pp. 201-205

Abstract

The existence of nicotinic acetylcholine receptors (nAChR) on lymphocytes remains controversial. We attempted to show the existence of nAChR on murine lymphocytes. The intraperitoneal injection of nicotine induced the lymphocytosis in the spleen on day 3. Although freshly isolated lymphocytes bound small quantities of fluorescein isothiocyanate (FITC)-conjugated alpha-bungarotoxin (alpha BuTx), they began to bind alpha BuTx after incubation in medium. In contrast to granulocytes, various lymphocyte subsets obtained from various lymphoid organs were found to bind alpha BuTx. Affinity purification of alpha BuTx-binding protein revealed that lymphocytes expressed the same nAChR molecules as those of muscle. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed that lymphocytes expressed the alpha-subunit mRNA ofnAChR. These results suggest that lymphocytes carry nAChR on the surface and are stimulated directly via their nAChR by parasympathetic nerve stimuli.

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Documento generato il 30/11/20 alle ore 07:14:25