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Titolo:
INFLUENCE OF 48 HOURS OF COLD-STORAGE IN UNIVERSITY-OF-WISCONSIN ORGAN PRESERVATION SOLUTION ON METABOLIC CAPACITY OF RAT HEPATOCYTES
Autore:
OLINGA P; MEREMA M; SLOOFF MJH; MEIJER DKF; GROOTHUIS GMM;
Indirizzi:
UNIV GRONINGEN,CTR PHARM,DEPT PHARMACOKINET & DRUG DELIVERY,GRONINGENINST DRUG STUDIES NL-9713 AV GRONINGEN NETHERLANDS UNIV HOSP,DEPT SURG,DIV HEPATOBILIARY SURG & LIVER TRANSPLANTAT GRONINGEN NETHERLANDS
Titolo Testata:
Journal of hepatology
fascicolo: 4, volume: 27, anno: 1997,
pagine: 738 - 743
SICI:
0168-8278(1997)27:4<738:IO4HOC>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
XENOBIOTIC METABOLISM; LIVER LOBULE; PERICENTRAL REGIONS; UW SOLUTION; VIABILITY; CYTOCHROME-P-450; 7-ETHOXYCOUMARIN; MAINTENANCE; TRANSPLANTATION; SUSPENSIONS;
Keywords:
DRUG METABOLISM; 7-ETHOXYCOUMARIN; HEPATOCYTES; LIDOCAINE; SHORT-TERM PRESERVATION; UW;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
P. Olinga et al., "INFLUENCE OF 48 HOURS OF COLD-STORAGE IN UNIVERSITY-OF-WISCONSIN ORGAN PRESERVATION SOLUTION ON METABOLIC CAPACITY OF RAT HEPATOCYTES", Journal of hepatology, 27(4), 1997, pp. 738-743

Abstract

Background/Aims: Suspensions of isolated hepatocytes are a valuable tool to study liver functions, For optimal use of the isolated hepatocytes, methods are needed to preserve the hepatocytes while maintaining their viability, metabolic and transport functions, Until now little has been known about the maintenance of the drug metabolism capacity and energy state, measured by the so-called energy charge (ATP+1/2ADP)/(ATP+ADP+AMP), in hepatocytes after storage in University of Wisconsin cold storage solution (UW), Consequently, we investigated whether UW, originally designed to preserve organs for transplantation, was suitable for preservation of isolated rat hepatocytes with respect to the maintenance of drug metabolism and levels of energy-rich substrates. Methods: Viability of the isolated rat hepatocytes was determined by trypan blue exclusion, ATP content and energy charge after 24 and 48 h of storage in UW at 0 degrees C, Phase I and II metabolic functions of the cells were studied by measuring the cytochrome P450 content and the metabolic rate of lidocaine and 7-ethoxycoumarin. Results: During 48 hof storage of hepatocytes in UW both phase I and phase II metabolism are preserved at control levels, After storage, the viability of the hepatocytes was not changed significantly, and the cells maintained proper cellular ATP content and overall energy charge. Conclusions: Theseresults imply that hepatocytes from a single isolation can be stored in UW solution and used for metabolism experiments for 3 consecutive days, allowing a reduction in the use of experimental animals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 10:53:27