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Titolo:
CALCIUM AND THE ANIONIC POLYPEPTIDE FRACTION (APF) HAVE OPPOSING EFFECTS ON CHOLESTEROL CRYSTALLIZATION IN MODEL BILE
Autore:
KONIKOFF FM; DELAPORTE PL; LAUFER H; DOMINGO N; LAFONT H; GILAT T;
Indirizzi:
ICHILOV HOSP,TEL AVIV MED CTR,DEPT GASTROENTEROL,6 WEIZMAN ST IL-64239 TEL AVIV ISRAEL TEL AVIV UNIV,SOURASKY TEL AVIV MED CTR,DEPT GASTROENTEROL IL-69978 TEL AVIV ISRAEL TEL AVIV UNIV,SACKLER FAC MED,MINERVA CTR CHOLESTEROL GALLSTONES & LIPID METAB IL-69978 TEL AVIV ISRAEL INSERM,U130 F-13258 MARSEILLE FRANCE
Titolo Testata:
Journal of hepatology
fascicolo: 4, volume: 27, anno: 1997,
pagine: 707 - 715
SICI:
0168-8278(1997)27:4<707:CATAPF>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIPOPROTEIN COMPLEX; BINDING PROTEIN; APOPROTEIN FRACTION; SUPERSATURATED BILE; GALLSTONE FORMATION; CRYSTAL NUCLEATION; PRECIPITATION; GROWTH; CARBONATE; SALT;
Keywords:
APF; BILE; CALCIUM; CHOLESTEROL; CRYSTALLIZATION; GALLSTONES; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
F.M. Konikoff et al., "CALCIUM AND THE ANIONIC POLYPEPTIDE FRACTION (APF) HAVE OPPOSING EFFECTS ON CHOLESTEROL CRYSTALLIZATION IN MODEL BILE", Journal of hepatology, 27(4), 1997, pp. 707-715

Abstract

Background/Aims: Cholesterol gallstones contain both calcium and biliary proteins, but their respective roles in gallstone pathogenesis areunknown, We have studied the effects of calcium and a major biliary protein, anionic polypeptide fraction, on the process of cholesterol crystallization in bile. Methods: Anionic polypeptide fraction was purified from human bile, Model bile composed of cholesterol, egg yolk lecithin and sodium taurocholate was prepared in a lipid concentration (18mM, 37 mM, and 120 mM, respectively) simulating lithogenic human gallbladder bile, The crystallization process was observed by phase contrast light microscopy, and sequential separation of precipitable cholesterol structures by sucrose density gradient ultracentrifugation. Results: Addition of calcium, or anionic polypeptide fraction alone, or both together did not influence the crystal observation time of bile (thetime which elapsed from initiation of supersaturation to the first appearance of crystals), However, the rate and quantity of cholesterol precipitation and crystal formation were affected by both, Calcium increased in a dose-dependent manner the cholesterol monohydrate crystal mass before apparent equilibrium was reached, This effect was inhibitedby anionic polypeptide fraction, which increased the amount of cholesterol within precipitable phospholipid vesicles, and decreased the rate of crystal formation, Fluorescence-labeled anionic polypeptide fraction revealed that anionic polypeptide fraction (with and without calcium) was primarily associated with vesicle aggregates. Conclusions: Ourdata demonstrate that calcium and anionic polypeptide fraction have opposing effects on the process of cholesterol crystallization and the resultant crystal mass without influencing the crystal observation time of bile, These findings suggest that biliary proteins, in addition to being crystallization effecters by themselves, may further influencecholesterol crystallization and gallstone formation by interacting with calcium and possibly other elements that coexist in bile.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 22:17:34