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Titolo:
DETECTION AND ENUMERATION OF ERWINIA-CAROTOVORA SUBSP ATROSEPTICA USING SPIRAL PLATING AND IMMUNOFLUORESCENCE COLONY STAINING
Autore:
SCHOBER BM; VANVUURDE JWL;
Indirizzi:
WAGENINGEN UNIV AGR,DEPT PHYTOPATHOL,POB 8025 NL-6700 EE WAGENINGEN NETHERLANDS RES INST PLANT PROTECT,INST PLANTEZIEKTENKUNDIG ONDERZOEK,DIENST LANDBOUWKUNDIG ONDERZOEK NL-6700 GW WAGENINGEN NETHERLANDS
Titolo Testata:
Canadian journal of microbiology
fascicolo: 9, volume: 43, anno: 1997,
pagine: 847 - 853
SICI:
0008-4166(1997)43:9<847:DAEOES>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIA; ROT; PSEUDOMONAS; SEED;
Keywords:
SOFT ROT; WITLOOF CHICORY; DETECTION; ERWINIA CAROTOVORA SUBSP ATROSEPTICA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
B.M. Schober e J.W.L. Vanvuurde, "DETECTION AND ENUMERATION OF ERWINIA-CAROTOVORA SUBSP ATROSEPTICA USING SPIRAL PLATING AND IMMUNOFLUORESCENCE COLONY STAINING", Canadian journal of microbiology, 43(9), 1997, pp. 847-853

Abstract

Immunofluorescence colony staining (IFC) and a new technique using spiral plating combined with IFC were evaluated for the soft-rot pathogen Erwinia carotovora subsp. atroseptica in witloof chicory. Target bacteria could be detected in platings at various dilutions of plant washings. Brilliance of the stained colonies of E. carotovora subsp. atroseptica was high. Spiral plating, used for both the plating of the bacteria and for the delivery of the conjugated antiserum, had a positive effect on the reduction of the background compared with the staining of the bacteria. The combination of spiral plating and IFC proved to bea functional tool for the quantification of target and nontarget bacteria and the isolation of target bacteria as pure culture from IFC-positive colonies. The method uses less conjugated antiserum than traditional IFC and produces results with very small variation within replications. The recovery of the bacteria in both pure culture and plant washing is significantly higher than the recovery using crystal violet pectate medium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 16:17:34