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Titolo:
RECONSTITUTED CL- PUMP PROTEIN - A NOVEL ION(CL-)-MOTIVE ATPASE
Autore:
GERENCSER GA; PURUSHOTHAM KR;
Indirizzi:
UNIV FLORIDA,COLL MED,DEPT PHYSIOL GAINESVILLE FL 32610 UNIV FLORIDA,COLL DENT,DEPT ORAL BIOL GAINESVILLE FL 32610
Titolo Testata:
Journal of bioenergetics and biomembranes
fascicolo: 6, volume: 28, anno: 1996,
pagine: 459 - 469
SICI:
0145-479X(1996)28:6<459:RCPP-A>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMA-MEMBRANE VESICLES; P-TYPE ATPASE; APLYSIA INTESTINE; TRANSLOCATING ATPASE; CHLORIDE TRANSPORT; RAT-BRAIN; SEQUENCE;
Keywords:
ION-MOTIVE ATPASE; CHLORIDE PUMP; CATALYTIC AND TRANSPORT KINETICS; ELECTROGENIC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
G.A. Gerencser e K.R. Purushotham, "RECONSTITUTED CL- PUMP PROTEIN - A NOVEL ION(CL-)-MOTIVE ATPASE", Journal of bioenergetics and biomembranes, 28(6), 1996, pp. 459-469

Abstract

Cl- absorption by the Aplysia californica foregut is effected throughan active Cl- transport mechanism located in the basolateral membraneof the epithelial absorptive cells. These basolateral membranes contain both Cl--stimulated ATPase and ATP-dependent Cl- transport activities which can be incorporated into liposomes via reconstitution. Utilizing the proteoliposomal preparation, it was demonstrated that ATP, andits subsequent hydrolysis, Mg2+, Cl-, and a pH optimum of 7.8 were required to generate maximal intraliposomal Cl- accumulation, electricalnegativity, and ATPase activity. Additionally, an inwardly-directed valinomycin-induced Kt diffusion potential, making the liposome interior electrically positive, enhanced both ATP-driven Cl- accumulation andelectrical potential while an outwardly-directed valinomycin-induced K+ diffusion potential, making the liposome interior electrically negative, decreased both ATP-driven Cl- accumulation and electrical potential compared with proteoliposomes lacking the ionophore. Either orthovanadate or p-chloromercurobenzene sulfonate inhibited both the ATP-dependent intraliposomal Cl- accumulation, intraliposomal negative potential difference, and also Cl--stimulated ATPase activity. Both aspects of Cl- pump transport kinetics and its associated catalytic component kinetics were the first obtained utilizing a reconstituted transporterprotein. These results strongly support the hypothesis that Cl--ATPase actively transports Cl- by an electrogenic process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 18:10:25