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Titolo:
IN-VITRO EFFECTS OF IL-12 AND IL-2 ON NK CELLS, CYTOKINE RELEASE AND CLONOGENIC ACTIVITY IN MYELODYSPLASTIC SYNDROMES (MDS)
Autore:
SARINA B; CORTELEZZI A; CATTANEO C; POMATI M; SILVESTRIS I; DISTEFANO M; LAMBERTENGHIDELILIERS D; HU C; MONZA M; MAIOLO AT;
Indirizzi:
UNIV MILAN,SERV EMATOL,OSPED MAGGIORE,IRCCS,VIA F SFORZA 35 I-20122 MILAN ITALY UNIV MILAN,SERV EMATOL,OSPED MAGGIORE,IRCCS I-20122 MILAN ITALY UNIV MILAN,IST MED INTERNA,OSPED MAGGIORE,IRCCS I-20122 MILAN ITALY
Titolo Testata:
Leukemia
fascicolo: 10, volume: 11, anno: 1997,
pagine: 1726 - 1731
SICI:
0887-6924(1997)11:10<1726:IEOIAI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
STIMULATORY FACTOR NKSF; NATURAL-KILLER-CELLS; CLONAL INVOLVEMENT; INTERFERON-GAMMA; LYMPHOCYTES-T; BONE-MARROW; INTERLEUKIN-12; PROLIFERATION; RESPONSES; ALPHA;
Keywords:
MYELODYSPLASTIC SYNDROMES; INTERLEUKIN-12; INTERLEUKIN-2; NK ACTIVITY; CLONOGENIC ACTIVITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
B. Sarina et al., "IN-VITRO EFFECTS OF IL-12 AND IL-2 ON NK CELLS, CYTOKINE RELEASE AND CLONOGENIC ACTIVITY IN MYELODYSPLASTIC SYNDROMES (MDS)", Leukemia, 11(10), 1997, pp. 1726-1731

Abstract

We evaluated the in vitro effects of IL-12, alone and in association with IL-2 on RIDS bone marrow and peripheral blood cells. Thirty-six patients and 14 healthy subjects were studied. Natural killer-activity (NK-a) levels and lymphocyte immunophenotypes were determined in freshbone marrow (BMMNC) and peripheral blood mononuclear cells (PBMNC), which then were resuspended in medium containing IL-2, IL-12 or IL-2 + IL-12 for 7 days. Re-evaluation of NK-a levels, lymphocyte immunophenotypes, clonogenic activity and cytokine release showed that, unlike IL-2, IL-12 did not significantly increase NK-a or CD3(-)/56(+) cell levels in either bone marrow or peripheral blood; IL-2 + 12 led to a significant increase that fell between the values reached by each cytokinealone. IL-2 + 12 and, although to a lesser extent, also IL-12 alone induced the release of large amounts of gamma-IFN and alpha-TNF. In addition, the number of clusters particularly decreased in the samples treated with IL-2 + 12 and IL-12 alone. Clonogenic activity was not modified after stimulation with any of the treatment. These data suggest that IL-12 induces the release of inhibitory cytokines in normal as well as MDS cells and that it could be used in patients with elevated bone marrow blastosis.

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Documento generato il 03/04/20 alle ore 04:17:51