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Titolo:
BRADYKININ-INDUCED CONTRACTION OF GUINEA-PIG LUNG IN-VITRO
Autore:
LACH E; TRIFILIEFF A; MOUSLI M; LANDRY Y; GIES JP;
Indirizzi:
UNIV LOUIS PASTEUR STRASBOURG 1,NEUROIMMUNOPHARMACOL PULM LAB,INSERM,CJF 9105,BP 24 F-67401 ILLKIRCH GRAFFENS FRANCE UNIV LOUIS PASTEUR STRASBOURG 1,NEUROIMMUNOPHARMACOL PULM LAB,INSERM,CJF 9105 F-67401 ILLKIRCH GRAFFENS FRANCE
Titolo Testata:
Naunyn-Schmiedeberg's archives of pharmacology
fascicolo: 2, volume: 350, anno: 1994,
pagine: 201 - 208
SICI:
0028-1298(1994)350:2<201:BCOGLI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
AIRWAY MICROVASCULAR LEAKAGE; INDUCED BRONCHOCONSTRICTION; SUBSTANCE-P; SMOOTH-MUSCLE; MAST-CELLS; RECEPTOR; INHIBITION; ACTIVATION; ANTAGONIST; RESPONSES;
Keywords:
BRADYKININ; GUINEA PIG LUNG PARENCHYMA; ISOLATED LUNG CELLS; PEPTIDASES; B-2 ANTAGONISTS; EICOSANOIDS; PHOSPHOINOSITIDES; INTRACELLULAR CALCIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
E. Lach et al., "BRADYKININ-INDUCED CONTRACTION OF GUINEA-PIG LUNG IN-VITRO", Naunyn-Schmiedeberg's archives of pharmacology, 350(2), 1994, pp. 201-208

Abstract

We have investigated the contractile Effect of bradykinin (BK) in guinea pig lung in vitro. BK induces a dose-related contraction of lung parenchymal strips which is increased significantly in the presence of 10(-5) M captopril (an angiotensin converting enzyme inhibitor) or 10(-5) M DL-thiorphan (a neutral endopeptidase inhibitor). The kininase Iinhibitor, DL-2-mercaptomethyl-3-guanidino-ethylthiopropionic acid (MGTPA), Ras no effect an the BK-induced contraction. BK is more potent in contracting parenchymal lung strips than other contractile agents (histamine, carbachol and substance P), how ever the BK-induced maximalcontraction is lower than those obtained with histamine and carbachol, The B-1 agonist, des-Arg(9)-BK, does not contract lung paren chymal strips. The new BK B-2 receptor antagonists (Hoe 140, NPC 17731 and NPC 17761), which possess binding affinities in the nanomolar range, inhibit the BK-induced coctractile response in a dose-dependent manner. The BK-induced contraction was unaffected by propranolol, atropine, tetrodotoxin, capsaicin pre-treatment, triprolidine, methylsergide, Ro 19-3704 and N omega-nitro-L-arginine-methyl-ester (L-NAME), excluding the involvement of nervous pathways, preformed mast cell mediators, platelet-activating factor and nitric oxide. However, indomethacin, a cyclooxygenase inhibitor, AA-861, a 5-lipoxygenase inhibitor, and furegrelate, a thromboxane A(2) synthase inhibitor, decreased. The contractileresponse to BK, suggesting that both cyclooxygenase and 5-lipoxygenase products are involved in this contraction, Thapsigargin, an inhibitor of the endoplasmic reticulum Ca2+ ATPase, abolished the BK-induced contraction demonstrating an intracellular calcium-dependent mechanism. Morever, on a mixed lung cell suspension, obtained by enzymatic digestion, BK is able to induce phosphoinositide production. We conclude that BK, acting on B-2 receptors, is a powerful contractile agent of theguinea pig lung in vitro. The BK-induced contraction, modulated by kininases II, is not dependent on neural mechanisms whereas both eicosanoids and intracellular calcium are involved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 11:23:45