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Titolo:
THE AFFINITY THRESHOLD FOR HUMAN B-CELL ACTIVATION VIA THE ANTIGEN RECEPTOR COMPLEX IS REDUCED UPON CO-LIGATION OF THE ANTIGEN RECEPTOR WITH CD21 (CR-2)
Autore:
MONGINI PKA; VILENSKY MA; HIGHET PF; INMAN JK;
Indirizzi:
HOSP JOINT DIS & MED CTR,DEPT RHEUMATOL,301 E 17TH ST NEW YORK NY 10003 NYU,SCH MED,KAPLAN COMPREHENS CANC CTR,DEPT PATHOL NEW YORK NY 10016 NIH,IMMUNOL LAB BETHESDA MD 20892
Titolo Testata:
The Journal of immunology
fascicolo: 8, volume: 159, anno: 1997,
pagine: 3782 - 3791
SICI:
0022-1767(1997)159:8<3782:TATFHB>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-DEPENDENT ANTIGEN; HUMAN LYMPHOCYTES-B; EPSTEIN-BARR VIRUS; COBRA VENOM FACTOR; IMMUNE-RESPONSE; MEMBRANE IGM; ANTIBODY-RESPONSE; CYCLE PROGRESSION; ACQUIRED-IMMUNITY; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
P.K.A. Mongini et al., "THE AFFINITY THRESHOLD FOR HUMAN B-CELL ACTIVATION VIA THE ANTIGEN RECEPTOR COMPLEX IS REDUCED UPON CO-LIGATION OF THE ANTIGEN RECEPTOR WITH CD21 (CR-2)", The Journal of immunology, 159(8), 1997, pp. 3782-3791

Abstract

The present studies have examined whether the potential of an Ag to co-ligate the complement (C3d)-binding CD21 receptor complex with the membrane IgM (mIgM) receptor complex can reduce the mIgM:Ag affinity threshold for triggering human B cell S phase entry. A series of Ab:dextran conjugates consisting of affinity-diverse anti-IgM mAb, with and without anti-CD21 mAb, were synthesized as polyclonally reactive, moderately multivalent ligands that mimic C3d-bearing and non-C3d-bearing Ag. Co-ligation of mIgM and CD21 significantly diminished both the ligand concentration threshold and the IgM:ligand affinity threshold for eliciting S phase entry in the presence of IL-4. Furthermore, such co-engagement ablated the triggering bonus associated with high mIgM:ligand affinity, suggesting that B cells with a high affinity for Ag are not preferentially activated over B cells of intermediate affinity upon encountering a multivalent Ag with bound C3d. The enhancing effects ofmIgM:CD21 co-ligation were restricted to law concentrations of ligand; at high concentrations, a decrease in B cell DNA synthesis was oftenobserved. The findings suggest that the ability of a moderately multivalent Ag substrate to engage B cells through both mIgM and CD21 is critical for B cell activation at limiting Ag concentrations, and furthermore, that mIgM:CD21 co-engagement may be particularly important in eliciting an immune response to such Ags in unprimed individuals in whom the majority of specific B cells are of low affinity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:07:36