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Titolo:
INTRACELLULAR ROUTES AND SELECTIVE RETENTION OF ANTIGENS IN MILDLY ACIDIC CATHEPSIN D LYSOSOME-ASSOCIATED MEMBRANE PROTEIN-1/MHC CLASS II-POSITIVE VESICLES IN IMMATURE DENDRITIC CELLS/
Autore:
LUTZ MB; ROVERE P; KLEIJMEER MJ; RESCIGNO M; ASSMANN CU; OORSCHOT VMJ; GEUZE HJ; TRUCY J; DEMANDOLX D; DAVOUST J; RICCIARDICASTAGNOLI P;
Indirizzi:
CNR,CELLULAR & MOL PHARMACOL CTR,VIA VANVITELLI 32 I-20129 MILAN ITALY CNR,CELLULAR & MOL PHARMACOL CTR I-20129 MILAN ITALY CNRS MARSEILLE LUMINY,INSERM,CTR IMMUNOL F-13288 MARSEILLE FRANCE UNIV UTRECHT,SCH MED,INST BIOMEMBRANES,DEPT CELL BIOL NL-3584 CX UTRECHT NETHERLANDS
Titolo Testata:
The Journal of immunology
fascicolo: 8, volume: 159, anno: 1997,
pagine: 3707 - 3716
SICI:
0022-1767(1997)159:8<3707:IRASRO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL LANGERHANS CELLS; COLONY-STIMULATING FACTOR; TUMOR-NECROSIS-FACTOR; MOUSE BONE-MARROW; T-CELLS; PROCESSED ANTIGEN; HUMAN MACROPHAGES; INVARIANT CHAIN; GROWTH-FACTOR; LYMPHOCYTE-B;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
M.B. Lutz et al., "INTRACELLULAR ROUTES AND SELECTIVE RETENTION OF ANTIGENS IN MILDLY ACIDIC CATHEPSIN D LYSOSOME-ASSOCIATED MEMBRANE PROTEIN-1/MHC CLASS II-POSITIVE VESICLES IN IMMATURE DENDRITIC CELLS/", The Journal of immunology, 159(8), 1997, pp. 3707-3716

Abstract

Immature dendritic cells (DC) use both macropinocytosis and mannose receptor-mediated endocytosis to internalize soluble Ags efficiently. These Ags are ultimately presented to T cells after DC maturation and migration into the lymph nodes. We have previously described the immortalized myeloid cell line FSDC as displaying the characteristics of early DC precursors that efficiently internalize soluble Ags. To describethe different routes of Ag uptake and to identify the Ag retention compartments in FSDC, we followed the intracellular fate of FITC-coupledOVA, dextran (DX), transferrin, and Lucifer Yellow using flow cytometry, confocal microscopy, and immunoelectron microscopy. OVA and DX gained access into macropinosomes and early endosomes. DX was preferentially sorted into endosomal compartments, while most of the OVA entered macropinosomes via fluid phase uptake. We found a long-lasting retention of DX and OVA of up to 24 h. After 6 h of chase, these two molecules were concentrated in common vesicular compartments. These retention compartments were distinct from endosomes and lysosomes; they were much larger, only mildly acidic, and lacked the small GTP binding proteinrab7. However, they were positive for lysosome-associated membrane protein-1, the protease cathepsin D, and MHC class II molecules, thus representing matured macropinosomes. These data suggest that the activity of vacuolar proteases is reduced at the mildly acidic pH of these vesicles, which explains their specific retention of an Ag. The retention compartments might be used by nonlymphoid tissue DC to store peripheral Ags during their migration to the lymph node.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 20:33:08