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Titolo:
IDENTIFICATION OF THE NUCLEOTIDES IN THE A-RICH BULGE OF THE TETRAHYMENA RIBOZYME RESPONSIBLE FOR AN EFFICIENT SELF-SPLICING REACTION
Autore:
IKAWA Y; OKADA A; IMAHORI H; SHIRAISHI H; INOUE T;
Indirizzi:
KYOTO UNIV,FAC SCI,DEPT CHEM,SAKYO KU KYOTO 60601 JAPAN KYOTO UNIV,FAC SCI,DEPT CHEM,SAKYO KU KYOTO 60601 JAPAN OSAKA UNIV,INST SCI & IND RES IBARAKI OSAKA 657 JAPAN
Titolo Testata:
Journal of Biochemistry
fascicolo: 4, volume: 122, anno: 1997,
pagine: 878 - 882
SICI:
0021-924X(1997)122:4<878:IOTNIT>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROUP-I INTRON; TERTIARY STRUCTURE; RIBOSOMAL-RNA; ESCHERICHIA-COLI; CATALYTIC ACTIVITY; FUNCTIONAL DOMAIN; SECONDARY; SEQUENCE; EXPRESSION; SELECTION;
Keywords:
A-RICH BULGE; IN VIVO SELECTION; P5ABC; RIBOZYME; TETRAHYMENA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
Y. Ikawa et al., "IDENTIFICATION OF THE NUCLEOTIDES IN THE A-RICH BULGE OF THE TETRAHYMENA RIBOZYME RESPONSIBLE FOR AN EFFICIENT SELF-SPLICING REACTION", Journal of Biochemistry, 122(4), 1997, pp. 878-882

Abstract

P5abc is a large extension of the PS element characteristic of subclasses IC1 and IC2 of group I introns. It has a conserved region termed the A-rich bulge, that is responsible for activation of the Tetrahymena self-splicing intron. By employing a modified color-colony assay system, we identified four adenosines in the bulge that are responsible for an efficient splicing reaction. On comparison with the,X-ray crystal structure of the P4-5-6 domains of the Tetrahymena intron, three adenosines at positions 183, 184, and 186 were found to be identical to those significantly contributing to the formation of its tertiary structure, However, our results show that an adenosine at 187 is involved in the formation of a Watson-Crick base pair with U135, although it forms a Hoogsteen base pair in the crystal structure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/06/20 alle ore 06:40:36