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Titolo:
REGULATED EXPRESSION OF FIBRONECTIN, LAMININ AND RELATED INTEGRIN RECEPTORS DURING THE EARLY CHONDROCYTE DIFFERENTIATION
Autore:
TAVELLA S; BELLESE G; CASTAGNOLA P; MARTIN I; PICCINI D; DOLIANA R; COLOMBATTI A; CANCEDDA R; TACCHETTI C;
Indirizzi:
FAC MED,IST ANAT UMANA GENOA ITALY FAC MED,IST ANAT UMANA GENOA ITALY FAC MED,DIPARTIMENTO ONCOL GENOA ITALY IST NAZL RIC CANC,CTR BIOTECNOL AVANZATE AVIANO ITALY IST ISTOL & EMBRIOL GEN AVIANO ITALY CRO AVIANO ITALY UNIV UDINE,DIPARTIMENTO SCI & BIOTECNOL BIOMED I-33100 UDINE ITALY
Titolo Testata:
Journal of Cell Science
, volume: 110, anno: 1997,
parte:, 18
pagine: 2261 - 2270
SICI:
0021-9533(1997)110:<2261:REOFLA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
BASEMENT-MEMBRANE COMPONENTS; HEPARIN-BINDING DOMAIN; LIMB-BUD; MESENCHYMAL CONDENSATION; INVITRO DEVELOPMENT; PATTERN-FORMATION; CELL-SURFACE; N-CADHERIN; HIND-LIMB; CHONDROGENESIS;
Keywords:
FIBRONECTIN; LAMININ; CHONDROGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
S. Tavella et al., "REGULATED EXPRESSION OF FIBRONECTIN, LAMININ AND RELATED INTEGRIN RECEPTORS DURING THE EARLY CHONDROCYTE DIFFERENTIATION", Journal of Cell Science, 110, 1997, pp. 2261-2270

Abstract

We have investigated the expression and localization of fibronectin, laminin, and their receptors, and we used an in vitro chick chondrocyte differentiation model to define a time hierarchy for their appearance in early chondrogenesis and to determine their role in the cell condensation process, By serum fibronectin depletion/reconstitution, or GRGDSP peptide competition experiments, we show that fibronectin contributes to the initial cell-cell interactions that occur during condensation, In later stages, a downregulation of both fibronectin and of its alpha(5) beta(1) integrin receptor occur, as demonstrated by mRNA and protein kinetics, Immunolocalisation studies suggest that the reduction of fibronectin in discrete areas is involved in local activation of the cell differentiation program, Furthermore, we show that laminin isexpressed during the in vitro cell condensation process in areas thatare negative for fibronectin staining, The types of laminin as well as the timing of expression have been determined by northern blot and RT-PCR analyses, The highest levels of expression are coincident with maximal cell aggregation, The alpha(3) beta(1) laminin receptor, highlyexpressed in dedifferentiated cells, follows later on the ligand trend. During in vitro chondrogenesis, a down-regulation in the B isoform,and an up-regulation of the A isoform, of the alpha subunit of the alpha(6) beta(1) laminin receptor occurs, Immunolocalisation studies suggest that laminin is involved in the definition of differentiating areas as opposed to non differentiating areas of the condensed region, i.e. the periphery, which eventually gives rise to the perichondrium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 00:16:26