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Titolo:
THE YEAST PRP3 PROTEIN IS A U4 U6 SNRNP PROTEIN NECESSARY FOR INTEGRITY OF THE U4/U6 SNRNP AND THE U4/U6.U5 TRI-SNRNP/
Autore:
ANTHONY JG; WEIDENHAMMER EM; WOOLFORD JL;
Indirizzi:
CARNEGIE MELLON UNIV,DEPT BIOL SCI,4400 5TH AVE PITTSBURGH PA 15213 CARNEGIE MELLON UNIV,DEPT BIOL SCI PITTSBURGH PA 15213
Titolo Testata:
RNA
fascicolo: 10, volume: 3, anno: 1997,
pagine: 1143 - 1152
SICI:
1355-8382(1997)3:10<1143:TYPPIA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMALL NUCLEAR RIBONUCLEOPROTEIN; RNA SPLICING INVITRO; SACCHAROMYCES-CEREVISIAE; PREMESSENGER RNA; S-CEREVISIAE; SPLICEOSOME; GENES; U4; BINDING; U6;
Keywords:
PRE-MESSENGER-RNA SPLICING; PRP3P; U4/U6 SNRNP PROTEIN; S-CEREVISIAE; SPLICEOSOME ASSEMBLY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
J.G. Anthony et al., "THE YEAST PRP3 PROTEIN IS A U4 U6 SNRNP PROTEIN NECESSARY FOR INTEGRITY OF THE U4/U6 SNRNP AND THE U4/U6.U5 TRI-SNRNP/", RNA, 3(10), 1997, pp. 1143-1152

Abstract

Previously, yeast prp3 mutants were found to be blocked prior to the first catalytic step of pre-mRNA splicing. No splicing intermediates or products are formed from pre-mRNA in heat-inactivated prp3 mutants or prp3 mutant extracts. Here we show that Prp3p is a component of the U4/U6 snRNP and is also present in the U4/U6.U5 tri-snRNP. Heat inactivation of prp3 extracts results in depletion of free U6 snRNPs and U4/U6.U5 tri-snRNPs, but not U4/U6 snRNPs or U5 snRNPs. Free U4 snRNP, normally not present in wild-type extracts, accumulates under these conditions. Assays of in vivo levels of snRNAs in a prp3 mutant revealed that amounts of free U6 snRNA decreased, free U4 snRNA increased, and U4/U6 hybrids decreased slightly. These results suggest that Prp3p is required for formation of stable U4/U6 snRNPs and for assembly of the U4/U6.U5 tri-snRNP from its component snRNPs. Upon inactivation of Prp3p, spliceosomes cannot assemble from prespliceosomes due to the absence of intact U4/U6.U5 tri-snRNPs. Prp3p is homologous to a human protein that is a component of U4/U6 snRNPs, exemplifying the conservation of splicing factors between yeast and metazoans.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:52:25