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Titolo:
PARTICIPATION OF CED-3 ICE AND YAMA PROTEINASES IN THE EXECUTION OF APOPTOSIS IN AK-5 TUMOR-CELLS LEADING TO SPONTANEOUS TUMOR-REGRESSION/
Autore:
KHAR A; PARDHASARADHI BVV; VARALAKSHMI C; ALI AM; KUMARI AL;
Indirizzi:
CTR CELLULAR & MOL BIOL,UPPAL RD HYDERABAD 500007 ANDHRA PRADESH INDIA
Titolo Testata:
Cellular immunology
fascicolo: 1, volume: 180, anno: 1997,
pagine: 29 - 35
SICI:
0008-8749(1997)180:1<29:POCIAY>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPONTANEOUS IMMUNOLOGICAL REGRESSION; FAS-MEDIATED CYTOTOXICITY; NATURAL-KILLER-CELLS; IL-1-BETA-CONVERTING ENZYME; POLY(ADP-RIBOSE) POLYMERASE; MAMMALIAN HOMOLOG; RAT HISTIOCYTOMA; DEATH; PROTEASE; BCL-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
A. Khar et al., "PARTICIPATION OF CED-3 ICE AND YAMA PROTEINASES IN THE EXECUTION OF APOPTOSIS IN AK-5 TUMOR-CELLS LEADING TO SPONTANEOUS TUMOR-REGRESSION/", Cellular immunology, 180(1), 1997, pp. 29-35

Abstract

AK-5, which is a spontaneously regressing rat histiocytoma, is killedby necrosis (perforin mediated) and apoptosis. We have studied the induction of apoptosis in AK-5 tumor cells by each of the following: a factor from anti-AK-5 antiserum, dexamethasone, and natural killer cells. Partial inhibition in apoptosis was observed when AK-5 cells were transfected with Crm A gene, a specific inhibitor of ICE protease. Similarly peptide inhibitors Ac-YVAD-cmk and Ac-DEVD-CHO inhibited partially the formation of nuclear bodies and DNA fragmentation induced by each of the above-mentioned apoptotic inducers. Although NR cells were able to kill Crm A and bcl-2 transfected clones by cytotoxic action, they failed to induce DNA fragmentation in these clones, suggesting a dual mode of action by NK cells in the induction of target cell death. We were unable to detect ICE and YAMA/CPP32 transcripts in control AK-5cells, but upon induction of the apoptotic process, there was significant expression of these transcripts in AK-5 cells. When bcl-2 gene was introduced into AK-5 cells there was complete inhibition of apoptosis, suggesting its affect to be upstream of ICE and YAMA proteases. These results suggest an important role for cysteine proteases in the execution of apoptosis, leading to tumor cell death and the regression ofAK-5 tumor in syngeneic hosts. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 00:38:27