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Titolo:
TRANSDUCTION POTENTIAL OF HUMAN RETROVIRUSES IN HIGHLY PROLIFERATING SMALL-CELL LUNG-CANCER CELLS AS WELL AS NONPROLIFERATING HEMATOPOIETICSTEM-CELLS
Autore:
HANSEN JES; GRAM GJ; NIELSEN SD; SORENSEN A; JENSEN PB; SEHESTED M; NIELSEN JO; RORTH M;
Indirizzi:
HVIDOVRE UNIV HOSP,DEPT 144,INFECT DIS LAB DK-2650 HVIDOVRE DENMARK RIGSHOSP,DEPT PATHOL DK-2100 COPENHAGEN DENMARK RIGSHOSP,DEPT ONCOL DK-2100 COPENHAGEN DENMARK
Titolo Testata:
APMIS. Acta pathologica, microbiologica et immunologica Scandinavica
fascicolo: 9, volume: 105, anno: 1997,
pagine: 723 - 729
SICI:
0903-4641(1997)105:9<723:TPOHRI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS INFECTION; GENE-THERAPY; HUMAN-SERUM; HIV-INFECTION; HTLV-I; VECTORS; CARCINOMA; DNA; NEUTRALIZATION; AMPLIFICATION;
Keywords:
RETROVIRUS; VECTOR; GENE THERAPY; SMALL-CELL LUNG CANCER; HEMATOPOIETIC STEM CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
J.E.S. Hansen et al., "TRANSDUCTION POTENTIAL OF HUMAN RETROVIRUSES IN HIGHLY PROLIFERATING SMALL-CELL LUNG-CANCER CELLS AS WELL AS NONPROLIFERATING HEMATOPOIETICSTEM-CELLS", APMIS. Acta pathologica, microbiologica et immunologica Scandinavica, 105(9), 1997, pp. 723-729

Abstract

Direct gene transfer to solid tissues or metastatic cancer cells requires vectors capable of in vivo transduction to specific cells. The predominant retroviral vectors of murine origin are inactivated by humancomplement, which precludes their use in vivo. Such inactivation doesnot take place with vectors based on human retroviruses. Murine retroviral vectors are also limited to proliferating cells, which human retroviruses are not. In this study we examined whether or not a vector using components from the human retroviruses HIV-1 and HTLV-1 could infect small-cell lung cancer cells and resting CD34+ hematopoietic stem cells. While HIV-1 itself was unable to infect cells lacking the CD4-membrane molecule, chimeric viral particles (pseudotype virus) with HIV-1 genome and HTLV-1 envelope components were able to infect both CD4-containing lymphocytic cells, CD4-negative tumour cells and hematopoietic stem cells. After infection with the pseudotype vector, the RNA genome was reverse transcribed and integrated. Transduction efficiency and gene expression under the HIV-1 LTR promoter in both tumour and stem cells were found to be of a similar or greater magnitude than in lymphocytic cells. These results suggest that gene transfer targeting proliferating as well as resting cells in vivo may be realized using components from human retroviruses.

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Documento generato il 04/12/20 alle ore 18:14:50