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Titolo:
STUDIES ON THE ROLE OF FIBROBLAST GROWTH-FACTOR SIGNALING IN NEUROGENESIS USING CONJUGATED AGED ANIMAL CAPS AND DORSAL ECTODERM-GRAFTED EMBRYOS/
Autore:
XU RH; KIM JB; TAIRA M; SREDNI D; KUNG HF;
Indirizzi:
NCI,FREDERICK CANC RES & DEV CTR,LAB BIOCHEM PHYSIOL,DIV BASIC SCI,BLDG 567,ROOM 152 FREDERICK MD 21702 NCI,FREDERICK CANC RES & DEV CTR,LAB BIOCHEM PHYSIOL,DIV BASIC SCI FREDERICK MD 21702 SCI APPLICAT INT CORP,INTRAMURAL RES SUPPORT PROGRAM FREDERICK MD 21702 UNIV TOKYO,GRAD SCH SCI,DEPT SCI BIOL,MOL EMBRYOL LAB TOKYO 113 JAPAN BAR ILAN UNIV,INTERDISCIPLINARY DEPT IL-52900 RAMAT GAN ISRAEL
Titolo Testata:
The Journal of neuroscience
fascicolo: 18, volume: 17, anno: 1997,
pagine: 6892 - 6898
SICI:
0270-6474(1997)17:18<6892:SOTROF>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
EARLY XENOPUS DEVELOPMENT; NEURAL INDUCTION; MESODERM INDUCTION; VENTRAL MESODERM; EARLY RESPONSE; HOMEOBOX GENE; MAP KINASE; EXPRESSION; FGF; GASTRULATION;
Keywords:
FGF; BMP; NEUROGENESIS; ANTEROPOSTERIOR PATTERNING; XENOPUS; EMBRYO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
R.H. Xu et al., "STUDIES ON THE ROLE OF FIBROBLAST GROWTH-FACTOR SIGNALING IN NEUROGENESIS USING CONJUGATED AGED ANIMAL CAPS AND DORSAL ECTODERM-GRAFTED EMBRYOS/", The Journal of neuroscience, 17(18), 1997, pp. 6892-6898

Abstract

Basic fibroblast growth factor (bFGF) has been shown to induce neuralfate in dissociated animal cap (AC) cells or in AC explants cultured in low calcium and magnesium concentrations. However, long-term disclosure of the cap may cause diffusion of the secreted molecule bone morphogenetic protein 4 (BMP-4), a neural inhibitor present in the AC. This may contribute to the subsequent neurogenesis induced by bFGF. Here we used conjugated and aged blastula AC to avoid diffusion of endogenous molecules from the AC. Unlike noggin, bFGF failed to induce neural tissue in this system. However, it enhanced neuralization elicited by a dominant negative BMP receptor (DN-BR) that inhibits the BMP-4 signaling. Posterior neural markers were turned on by bFGF in AC expressingDN-BR or chordin. Blocking the endogenous FGF signal with dominant negative FGF receptor (XFD) mainly inhibited development of posterior neural tissue in neuralized ACs. These in vitro studies were confirmed in vivo in embryos grafted with XFD-expressing ACs in the place of neuroectoderm. Expression of some regional neural markers was inhibited, although markers for muscle and posterior notochord were still detectable in the grafted embryos, suggesting that XFD specifically affected neurogenesis but not the dorsal mesoderm. The use of these in vitro andin vivo model systems provides new evidence that FGF, although unableto initiate neurogenesis on its own, is required for neural inductionas well as for posteriorization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 04:23:27