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Titolo:
CONSTITUTIVE EXPRESSION OF BETA-N-ACETYLHEXOSAMINIDASE IN A MICROGLIAL CELL-LINE - TRANSCRIPTIONAL MODULATION BY LIPOPOLYSACCHARIDE AND SERUM FACTORS
Autore:
BECCARI T; ORLACCHIO A; COSTANZI E; APPOLLONI MG; LAURENZI A; BOCCHINI V;
Indirizzi:
UNIV PERUGIA,DIPARTIMENTO BIOL CELLULARE & MOL,VIA GIOCHETTO I-06126 PERUGIA ITALY UNIV PERUGIA,DIPARTIMENTO MED CLIN PATOL & FARMACOL I-06100 PERUGIA ITALY
Titolo Testata:
Journal of neuroscience research
fascicolo: 1, volume: 50, anno: 1997,
pagine: 44 - 49
SICI:
0360-4012(1997)50:1<44:CEOBIA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEQUENCE-ANALYSIS; MOUSE-TISSUES; HEXOSAMINIDASE; 6-SULFATE; CLONING; SUBUNIT; ENZYME;
Keywords:
MICROGLIA; HEXOSAMINIDASE; DEAE CELLULOSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
T. Beccari et al., "CONSTITUTIVE EXPRESSION OF BETA-N-ACETYLHEXOSAMINIDASE IN A MICROGLIAL CELL-LINE - TRANSCRIPTIONAL MODULATION BY LIPOPOLYSACCHARIDE AND SERUM FACTORS", Journal of neuroscience research, 50(1), 1997, pp. 44-49

Abstract

We investigated the expression of the alpha- and beta-subunits of thelysosomal enzyme beta-N-acetylhexosaminidase in the BV-2 microglial cell line under different culture conditions, beta-N-acetylhexosaminidase from BV-2 microglia cells was separated into its constituent isoenzymes on diethylaminoethyl (DEAE) cellulose, and its activity was monitored with 4-methylumbelliferyl-beta-N-acetylglucosamine and 4-methylumbelliferyl-beta-N-acetylglucosamine substrates, Forms corresponding tothe mouse isoenzymes A and B were present in the cells incubated in serum-supplemented medium as well as in serum-free medium, Lipopolysaccharide, a well-known activator of microglia in vitro, added to the BV-2 cells in serum-supplemented medium induced a decrease in the specific enzymatic activity determined with the 4-methylumbelliferyl-beta-N-acetylglucosamine substrate, Lipopolysaccharide had no effect on hexosaminidase isoenzyme pattern of BV-2 cells in serum-supplemented medium. The level of alpha-subunit mRNA was increased and the level of beta-subunit mRNA was decreased in BV-2 cells incubated in serum-supplemented medium plus lipopolysaccharide, In the cells incubated in a serum-free medium no significant changes in the hexosaminidase-specific activities towards the above substrates were observed, Interestingly, increased expression of alpha- and beta-subunit mRNA was evident in comparison with cultures in serum-supplemented medium, The present results suggest that the BV-2 cell line may be a useful tool to study the possible role of microglia in the metabolism of brain glycolipids. (C) 1997 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 15:37:41