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Titolo:
IMAGING SUPRAMOLECULAR AGGREGATES IN BILE MODELS AND HUMAN BILE
Autore:
KAPLUN A; KONIKOFF FM; EITAN A; RUBIN M; VILAN A; LICHTENBERG D; GILAT T; TALMON Y;
Indirizzi:
TEL AVIV MED CTR & SCH MED,DEPT GASTROENTEROL,6 WEIZMAN ST IL-64239 TEL AVIV ISRAEL TEL AVIV MED CTR & SCH MED,DEPT GASTROENTEROL IL-64239 TEL AVIV ISRAEL TECHNION ISRAEL INST TECHNOL,DEPT CHEM ENGN IL-32000 HAIFA ISRAEL NAHARIYA HOSP,DEPT SURG NAHARIYYA ISRAEL BEILINSON MED CTR,DEPT SURG A IL-49100 PETAH TIQWA ISRAEL TEL AVIV UNIV,SACKLER FAC MED,DEPT PHYSIOL & PHARMACOL IL-69978 TEL AVIV ISRAEL
Titolo Testata:
Microscopy research and technique
fascicolo: 1, volume: 39, anno: 1997,
pagine: 85 - 96
SICI:
1059-910X(1997)39:1<85:ISAIBM>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSMISSION ELECTRON-MICROSCOPY; CHOLESTEROL GALLSTONE DISEASE; VESICLE-MICELLE TRANSITION; SUPERSATURATED BILE; CRYSTALLIZATION; CARRIERS; PHOSPHATIDYLCHOLINE; NUCLEATION; SYSTEMS; SOLUBILIZATION;
Keywords:
CHOLESTEROL; CRYSTALLIZATION; BILE; GALLSTONE FORMATION; CRYO-TEM; MICELLE; VESICLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
A. Kaplun et al., "IMAGING SUPRAMOLECULAR AGGREGATES IN BILE MODELS AND HUMAN BILE", Microscopy research and technique, 39(1), 1997, pp. 85-96

Abstract

Investigation of cholesterol crystallization is essential for the understanding of gallstone formation. Previous work has revealed a variety of aggregates of different sizes and shapes prior to the appearance of ''classical'' plate-like cholesterol monohydrate crystals both in native biles and model systems. In this article, we review existing data based on various microscopic techniques and present data on microstructural pathways leading to cholesterol crystal formation in two different bile models and in native bile. In continuation of our recent investigation of the microstructures in nucleating human bile, we now present data suggesting that polymorphism is not limited to complex native bile, but also appears in two, simplified model systems. These studies employed cryo-transmission electron microscopy (cryo-TEM) and video-enhanced light microscopy, using Nomarski optics (VELM). Only the combined use of these two complementary, non-perturbing direct methods can cover the whole range of microstructures ranging from a few nanometers to several microns. Concentrated isotropic solutions of bile models, composed of cholesterol, lecithin and taurocholate, were diluted to induce cholesterol supersaturation and start an evolution of microstructures, leading to cholesterol crystallization. Initially, small spheroidal micelles were observed by cryo-TEM. Subsequently, uni-, oligo-and multilamellar vesicles, compatible with structures seen at the same time by VELM, appeared in coexistence with micelles. Thereafter, during a dynamic phase of cholesterol crystallization, filaments, tubular and helical microstructures, as well as classical plate-like cholesterol monohydrate crystals were noted by light microscopy, Eventually, large plate-like crystals were observed by VELM, while cryo-TEM revealed only small spheroidal micelles. The crystallization process in native human bile during ex vivo incubation was found to bear close resemblance to the findings in the model systems, further supporting the applicability of these systems to the exploration of microstructural aspectsof nucleating human bile. (C) 1997 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 10:04:46