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Titolo:
THE ROLE OF CHONDROITIN SULFATE CHAINS OF URINARY TRYPSIN-INHIBITOR IN INHIBITION OF LPS-INDUCED INCREASE OF CYTOSOLIC-FREE CA2+ IN HL60 CELLS AND HUVEC CELLS
Autore:
KANAYAMA N; MAEHARA K; SUZUKI M; FUJISE Y; TERAO T;
Indirizzi:
HAMAMATSU UNIV,SCH MED,DEPT OBSTET & GYNECOL,3600 HANDA CHO HAMAMATSUSHIZUOKA 43131 JAPAN HAMAMATSU UNIV,SCH MED,DEPT CHEM HAMAMATSU SHIZUOKA 43131 JAPAN HAMAMATSU UNIV,SCH MED,EQUIPMENT CTR HAMAMATSU SHIZUOKA 43131 JAPAN
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 2, volume: 238, anno: 1997,
pagine: 560 - 564
SICI:
0006-291X(1997)238:2<560:TROCSC>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTER-ALPHA-INHIBITOR; IMMUNOELECTROPHORESIS; PLASMA; ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
N. Kanayama et al., "THE ROLE OF CHONDROITIN SULFATE CHAINS OF URINARY TRYPSIN-INHIBITOR IN INHIBITION OF LPS-INDUCED INCREASE OF CYTOSOLIC-FREE CA2+ IN HL60 CELLS AND HUVEC CELLS", Biochemical and biophysical research communications, 238(2), 1997, pp. 560-564

Abstract

Preincubation of HL60 cells and HUVEC cells with urinary trypsin inhibitor (UTI) inhibited increase of cytosolic free Ca2+ induced by LPS. In contrast, an increase of cytosolic free Ca2+ induced by LPS was notinhibited by deglycosylated UTI, UTI treated with monoclonal antibodyof chondroitin sulfate, Ca-45(2+) binding showed that UTI binds Ca-45(2+) dose-dependently, Scatchard plot analysis showed that UTI has twobinding sites for Ca2+, a high affinity binding site (Kd=15 mu M) anda low affinity site (Rd=150 mu M), and that UTI has more than 70 Ca2binding sites per molecule, The Ca2+ binding capacity of deglycosylated UTI and UTI treated with monoclonal antibody of chondroitin sulfatewas markedly depressed, Furthermore, UTI forms multi-polymers in the presence of Ca2+ as demonstrated by gel filtration and agarose gel electrophoresis, These results suggest that UTI is a physiological Ca2+ chelator on the cells and that the action is due to chondroitin sulfatechains of UTI. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 18:30:32