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Titolo:
IFN-GAMMA GENE-TRANSFER RESTORES HLA-CLASS-I EXPRESSION AND MAGE-3 ANTIGEN PRESENTATION TO CTL IN HLA-DEFICIENT SMALL-CELL LUNG-CANCER
Autore:
TRAVERSARI C; MEAZZA R; COPPOLECCHIA M; BASSO S; VERRECCHIA A; VANDERBRUGGEN P; ARDIZZONI A; GAGGERO A; FERRINI S;
Indirizzi:
IST NAZL RIC CANC,CBA,LARGO BENZI 10 I-16132 GENOA ITALY IST NAZL RIC CANC,CBA I-16132 GENOA ITALY DIBIT HS RAFFAELE MILAN ITALY UNIV GENOA,DIPARTIMENTO ONCOL SPERIMENTALE & CLIN GENOA ITALY LUDWIG INST CANC RES,BRUSSELS BRANCH BRUSSELS BELGIUM
Titolo Testata:
Gene therapy
fascicolo: 10, volume: 4, anno: 1997,
pagine: 1029 - 1035
SICI:
0969-7128(1997)4:10<1029:IGRHEA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOLYTIC T-LYMPHOCYTES; MURINE MAMMARY ADENOCARCINOMA; TUMOR-NECROSIS-FACTOR; IL-2 GENE; MELANOMA; IDENTIFICATION; REGRESSION; CARCINOMA; THERAPY; LINES;
Keywords:
INTERFERON-GAMMA; ANTIGEN PRESENTATION; TUMOR-ASSOCIATED ANTIGENS; CYTOLYTIC T LYMPHOCYTES; SMALL CELL LUNG CANCER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
C. Traversari et al., "IFN-GAMMA GENE-TRANSFER RESTORES HLA-CLASS-I EXPRESSION AND MAGE-3 ANTIGEN PRESENTATION TO CTL IN HLA-DEFICIENT SMALL-CELL LUNG-CANCER", Gene therapy, 4(10), 1997, pp. 1029-1035

Abstract

In this study, we have analyzed the possibility of inducing I cell responses against small cell lung cancer (SCLC), a still incurable tumor, by cytokine gene transfer approaches. By RT-PCR analysis most SCLC expressed the CTL-defined tumor antigens MAGE-3 (10/11), MAGE-1 (7/11) and less frequently BAGE (4/11) and GAGE1,2 (4/11). Although the surface expression of HLA class I molecules was low on most SCLC, thus preventing CTL recognition, treatment of the cells with IFN-gamma enhancedHLA-class I levels in all cases. Two MAGE3(+) SCLC cell lines displaying the A2 HLA-class I allele, involved in MAGE-3 antigen presentationto CTL, were stably transfected with the IFN-gamma gene (alone or co-transfected with IL-2). IFN-gamma-transfected cells displayed a clearcut increase in expression of HLA-class I and beta(2) microglobulin at both protein and mRNA level and of TAP-1 and TAP-2 mRNA. Perhaps more importantly, IFN-gamma transfected cells were recognized by the MAGE-3-specific, A2-restricted antimelanoma CTL clone 297/22, while unmodified cells or cells transfected with the IL-2 gene alone were not. Thesedata indicate that IFN-gamma gene transfection into HLA-deficient SCLC cells is able to restore their ability to present endogenous tumor antigens to CTL and that IFN-gamma gene transfer approaches may be attempted to induce specific CTL responses in SCLC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:27:46