Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
LYSOSOMAL ALPHA-MANNOSIDASES OF MOUSE-TISSUES - CHARACTERISTICS OF THE ISOENZYMES, AND CLONING AND EXPRESSION OF A FULL-LENGTH CDNA
Autore:
BECCARI T; APPOLLONI MG; COSTANZI E; STINCHI S; STIRLING JL; DELLAFAZIA MA; SERVILLO G; VIOLA MP; ORLACCHIO A;
Indirizzi:
UNIV PERUGIA,DIPARTIMENTO BIOL CELLULARE & MOL,VIA GIOCHETTO I-06126 PERUGIA ITALY UNIV LONDON KINGS COLL,DIV LIFE SCI LONDON W8 7AH ENGLAND UNIV PERUGIA,MONTELUCE POLICLIN,IST PATOL GEN I-06126 PERUGIA ITALY
Titolo Testata:
Biochemical journal
, volume: 327, anno: 1997,
parte:, 1
pagine: 45 - 49
SICI:
0264-6021(1997)327:<45:LAOM-C>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
T. Beccari et al., "LYSOSOMAL ALPHA-MANNOSIDASES OF MOUSE-TISSUES - CHARACTERISTICS OF THE ISOENZYMES, AND CLONING AND EXPRESSION OF A FULL-LENGTH CDNA", Biochemical journal, 327, 1997, pp. 45-49

Abstract

Lysosomal alpha-D-mannosidase from mouse tissues was separated into its constituent isoenzymes by DEAE-cellulose chromatography. Forms corresponding to the human isoenzymes B and A were present in testis, brain, spleen and kidney, whereas in epididymis and liver only the B form was present. Murine alpha-mannosidases A and B are glycoproteins and have pH optima, thermal stabilities and molecular masses similar to those of the human isoenzymes. A full-length cDNA. (3.1 kb) containing the complete coding sequence for alpha-mannosidase was isolated from a mouse macrophage cDNA library. Comparison of the deduced amino acid sequences of human and mouse alpha-mannosidases showed that they had 75% identity and 83% similarity. Expression of this cDNA in COS cells showed that both the A and the B isoenzymes can arise from a single transcript. Northern blotting analysis showed a 10-fold range in the abundance of alpha-mannosidase mRNA in mouse tissues, with the highest levelsfound in epididymis, and the lowest in liver.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 09:13:25