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Titolo:
INFLUENCE OF SURFACE HYDROPHILIC HYDROPHOBIC BALANCE ON ENZYME PROPERTIES/
Autore:
LONGO MA; COMBES D;
Indirizzi:
INST NATL SCI APPL,CTR BIOINGN GILBERT DURAND,URA CNRS 544,COMPLEXE SCI RANGUEIL F-31077 TOULOUSE FRANCE INST NATL SCI APPL,CTR BIOINGN GILBERT DURAND,URA CNRS 544 F-31077 TOULOUSE FRANCE
Titolo Testata:
Journal of biotechnology
fascicolo: 1, volume: 58, anno: 1997,
pagine: 21 - 32
SICI:
0168-1656(1997)58:1<21:IOSHHB>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID ESTERIFICATION; GLYCOL-MODIFIED ENZYMES; CANDIDA-RUGOSA LIPASE; POLYETHYLENE-GLYCOL; ALPHA-CHYMOTRYPSIN; PEPTIDE-SYNTHESIS; ORGANIC-SOLVENTS; SOLUBLE POLYSACCHARIDES; COVALENT ATTACHMENT; ESTER SYNTHESIS;
Keywords:
ENZYME MODIFICATION; GLYCOSYLATION; PEG; HYDROPHILIC/HYDROPHOBIC; STABILITY; ACTIVITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
M.A. Longo e D. Combes, "INFLUENCE OF SURFACE HYDROPHILIC HYDROPHOBIC BALANCE ON ENZYME PROPERTIES/", Journal of biotechnology, 58(1), 1997, pp. 21-32

Abstract

Two different enzyme surface modifications were carried out in order to alter the protein hydrophilic/hydrophobic balance in opposite directions and to observe the effects induced on enzyme properties. First, a novel chemoenzymatic glycosylation method was applied, which resulted in a higher enzyme surface hydrophilic character. Then, an amphiphilic polymer, PEG, was bound to the enzymes by chemical means, and it brought about an increase in the global hydrophobic character. Two different enzymes, a-chymotrypsin and Candida rugosa lipase, were studied, and in all cases, several degrees of modification were obtained. Then,the modified biocatalysts were thoroughly investigated, and the influence of the variation of surface hydrophilic/hydrophobic balance on hydrolytic activity, hydrolysis kinetic parameters, synthetic activity and thermal stability was assessed. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 12:39:07