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Titolo:
DISTINCT INDUCTION OF C-FOS MESSENGER-RNA IN NG108-15 CELLS TRANSFECTED WITH MUSCARINIC M1 AND M3 RECEPTORS
Autore:
TOHDA M; TOHDA C; SAKUMA S; HIGASHIDA H; NOMURA Y;
Indirizzi:
HOKKAIDO UNIV,FAC PHARMACEUT SCI,DEPT PHARMACOL SAPPORO HOKKAIDO 060 JAPAN HOKKAIDO UNIV,FAC PHARMACEUT SCI,DEPT PHARMACOL SAPPORO HOKKAIDO 060 JAPAN KANAZAWA UNIV,SCH MED,NEUROINFORMAT RES INST,DEPT BIOPHYS KANAZAWA ISHIKAWA 920 JAPAN TOYAMA MED & PHARMACEUT UNIV,ORIENTAL MED WAKAN YAKU RES INST,DEPT NEUROSCI TOYAMA 93001 JAPAN
Titolo Testata:
European journal of pharmacology. Molecular pharmacology section
fascicolo: 2, volume: 268, anno: 1994,
pagine: 169 - 176
SICI:
0922-4106(1994)268:2<169:DIOCMI>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; GLIOMA HYBRID-CELLS; GROWTH-FACTOR; ACETYLCHOLINE-RECEPTORS; BINDING-PROPERTIES; SWISS 3T3-CELLS; PHORBOL ESTER; MESSENGER-RNA; SUBTYPES; CALCIUM;
Keywords:
MUSCARINIC ACETYLCHOLINE RECEPTOR; POLYPHOSPHOINOSITIDE TURNOVER; C-FOS EXPRESSION; CALMODULIN; PROTEIN KINASE C; NG108-15 CELL, TRANSFECTED;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
M. Tohda et al., "DISTINCT INDUCTION OF C-FOS MESSENGER-RNA IN NG108-15 CELLS TRANSFECTED WITH MUSCARINIC M1 AND M3 RECEPTORS", European journal of pharmacology. Molecular pharmacology section, 268(2), 1994, pp. 169-176

Abstract

The differences of intracellular signalling mechanisms between muscarinic acetylcholine m1 and m3 receptors, which are coupled with polyphosphoinositide turnover, were examined by using m1- and m3-transfected NG108-15 cells. The c-fos mRNA was induced by 1 mM acetylcholine peak at 60 min in both m1 and m3 cells. The c-fos induction in m1 cells wasinhibited by 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'- acid tetraacetoxymethyl ester (BAPTA-AM) and N-(6-aminohexyl)dichloro-1-naphthalenesulfonamide hydrochloride (W-7), but was not inhibited by prolonged treatment with 12-O-tetradecanoylphorbol 13-acetate (TPA), suggesting thatintracellular Ca2+ and calmodulin are involved in the induction. The c-fos induction in m3 cells was inhibited by BAPTA-AM and prolonged treatment with TPA, but was not influenced by W-7, suggesting that protein kinase C is mainly involved in m3-induced c-fos expression. Acetylcholine induced an increase in inositol phosphates and a transient increase in the intracellular concentration of Ca2+ in both m1 and m3 cells. Sustained stimulation of acetylcholine strongly increased the inositol monophosphate content in m3 cells, but that of inositol trisphosphate and inositol diphosphate in m1 cells. These results suggest that the difference between m1- and m3-induced c-fos mRNA induction mechanisms is due to the difference in respective properties in polyphosphoinositide turnover.

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Documento generato il 29/09/20 alle ore 20:46:35