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Titolo:
SKELETAL AND CARDIAC ALPHA-ACTIN ISOFORMS EXHIBIT UNANTICIPATED TEMPORAL AND TISSUE-SPECIFIC GENE-EXPRESSION PATTERNS IN DEVELOPING AVIAN LIMBS AND EMBRYOS
Autore:
LINJONES J; HAUSCHKA SD;
Indirizzi:
UNIV WASHINGTON,DEPT BIOCHEM,BOX 357350 SEATTLE WA 98195 UNIV WASHINGTON,DEPT BIOCHEM SEATTLE WA 98195 UNIV WASHINGTON,DEPT ZOOL SEATTLE WA 98195
Titolo Testata:
Developmental biology
fascicolo: 2, volume: 189, anno: 1997,
pagine: 322 - 334
SICI:
0012-1606(1997)189:2<322:SACAIE>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERUM RESPONSE FACTOR; NUCLEOTIDE-SEQUENCE; MESSENGER-RNAS; 3'-UNTRANSLATED REGIONS; NUCLEAR FACTOR; MOUSE MUSCLE; LEG BUDS; DIFFERENTIATION; TRANSCRIPTION; PROMOTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
J. Linjones e S.D. Hauschka, "SKELETAL AND CARDIAC ALPHA-ACTIN ISOFORMS EXHIBIT UNANTICIPATED TEMPORAL AND TISSUE-SPECIFIC GENE-EXPRESSION PATTERNS IN DEVELOPING AVIAN LIMBS AND EMBRYOS", Developmental biology, 189(2), 1997, pp. 322-334

Abstract

The initial expression of skeletal muscle structural genes typically occurs after myogenic determination factor gene expression. We investigated this temporal relationship via a reverse transcription-polymerase chain reaction (RT-PCR) analysis of skeletal and cardiac alpha-actin(s- and c-actin) mRNA during chick limb development. c-actin transcripts were first detected at the beginning of muscle cytodifferentiationin stage 24/25 limbs, shortly after the initial appearance of MyoD and myogenin mRNAs, and were not detected in nonmyogenic tissues. In contrast, s-actin mRNA was detected in limbs at stage 15-16, periods whenmyf5 and MRF4 but not MyoD and myogenin transcripts are present (Lin-Tones, J., and Hauschka, S. D., Dev. Biol. 174, 407-422, 1996). While s-actin mRNA was not detected in E7 neural retina and was at variable levels in stage 9-15 neural tube, significant levels were consistentlydetected in mesodermal tissues which contribute nonmyogenic cells to the limb: stage 9-12 lateral plate and distal portions of stage 25/26 limbs. s-actin transcripts detected in the earliest limbs could thus be in myogenic and/or nonmyogenic cells. These data indicate that whilec-actin expression is activated at the onset of limb muscle cytodifferentiation, s-actin expression occurs much earlier, as well as in somenonmyogenic tissues. Whether the precocious expression of s-actin plays a functional role in muscle development remains to be determined. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 14:33:20