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Titolo:
CELLULAR MOTILITY IN-VITRO AS REVEALED BY SCANNING ACOUSTIC MICROSCOPY DEPENDS ON CELL-CELL CONTACTS
Autore:
ZOLLER J; BRANDLE K; BEREITERHAHN J;
Indirizzi:
UNIV FRANKFURT,CINEMAT CELL RES GRP,MARIE CURIE STR 9 D-60439 FRANKFURT GERMANY UNIV FRANKFURT,CINEMAT CELL RES GRP D-60439 FRANKFURT GERMANY UNIV FRANKFURT,INST ZOOL D-6000 FRANKFURT GERMANY
Titolo Testata:
Cell and tissue research
fascicolo: 1, volume: 290, anno: 1997,
pagine: 43 - 50
SICI:
0302-766X(1997)290:1<43:CMIARB>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
BEHAVIOR; CULTURE; FIBROBLASTS; EXPRESSION; PATTERNS; GROWTH;
Keywords:
CELL MOTILITY; CONTACT INHIBITION; SCANNING ACOUSTIC MICROSCOPY; EPITHELIAL CELLS; FIBROBLASTS; TISSUE CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
J. Zoller et al., "CELLULAR MOTILITY IN-VITRO AS REVEALED BY SCANNING ACOUSTIC MICROSCOPY DEPENDS ON CELL-CELL CONTACTS", Cell and tissue research, 290(1), 1997, pp. 43-50

Abstract

A new method is described for observing and quantifying an aspect of contact inhibition of cell movement that is sometimes called ''contactparalysis''. Based on the scanning acoustic microscope (SAM), the method can detect changes in the mechanical properties of cells, as well as changes in their motility and may therefore be more sensitive to some dynamic changes than methods based on optical microscopy. With thismethod intracellular motility of normal and transformed cells of epithelial and fibroblastic oirigin was investigated. By subtraction of SAM images patterns of motility, domains were detected that changed in acharacteristic way among various cell lines. Wave-like and nucleatingdomains could be distinguished; they were also used for the quantification of motility. Like migration intracellular motility is influencedby cell-cell contacts. In zones where a cell touches its neighbours motility domains change or dis appear depending on the cell type. In immortalized epithelial cells (XTH-2 cells) large quiescent zones developed in the region where contact with neighbouring cells was established, whereas in fibroblastic (3T3) cells motility was reduced less and did not change in domain pattern. Epithelial and fibroblastic cells were less motile when in contact with other cells or in confluent cultures than when solitary, i.e. their motility was contact inhibit ed. Transformed (SV40 3T3) cells, however, did not reduce their motility when in contact to or enclosed by other cells. The molecular basis for motility domains remains to be investigated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 09:09:38