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Titolo:
INTERLEUKIN-2 PROMOTER ENHANCER CONTROLLED EXPRESSION OF A SYNTHETIC CECROPIN-CLASS LYTIC PEPTIDE IN TRANSGENIC MICE AND SUBSEQUENT RESISTANCE TO BRUCELLA-ABORTUS/
Autore:
REED WA; ELZER PH; ENRIGHT FM; JAYNES JM; MORREY JD; WHITE KL;
Indirizzi:
LOUISIANA STATE UNIV,DEPT BIOCHEM BATON ROUGE LA 70803 LOUISIANA STATE UNIV,DEPT BIOCHEM BATON ROUGE LA 70803 UTAH STATE UNIV,DEPT ANIM DAIRY & VET SCI LOGAN UT 84322 UTAH STATE UNIV,CTR BIOTECHNOL 201 LOGAN UT 84322 LOUISIANA STATE UNIV,DEPT VET SCI BATON ROUGE LA 70803
Titolo Testata:
Transgenic research
fascicolo: 5, volume: 6, anno: 1997,
pagine: 337 - 347
SICI:
0962-8819(1997)6:5<337:IPECEO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRYPTOSPORIDIUM-PARVUM; ANTIBACTERIAL ACTIVITY; ANTIMICROBIAL PEPTIDE; MURINE INTERLEUKIN-2; OVINE INTERLEUKIN-2; AMPHIPATHIC HELIX; MOLECULAR-CLONING; GENE-EXPRESSION; INSECT IMMUNITY; DNA-POLYMERASE;
Keywords:
BRUCELLOSIS; CECROPIN; GENE THERAPY; INTERLEUKIN 2; SHIVA 1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
W.A. Reed et al., "INTERLEUKIN-2 PROMOTER ENHANCER CONTROLLED EXPRESSION OF A SYNTHETIC CECROPIN-CLASS LYTIC PEPTIDE IN TRANSGENIC MICE AND SUBSEQUENT RESISTANCE TO BRUCELLA-ABORTUS/", Transgenic research, 6(5), 1997, pp. 337-347

Abstract

The addition of an antimicrobial that can be synthesized by the mammalian immune system at the point of challenge may enhance disease resistance. A possible group of agents are cecropins, broad-spectrum antimicrobial peptides, which have been described and characterized. They are relatively non-toxic to normal cells from multicellular organisms but are toxic to a wide range of bacteria, protozoa and fungi, as well as infected and abnormal cells. Twenty-six lines of transgenic mice were produced by pronuclear injection of DNA consisting of the 5'-flanking region from -593 to +110 of the mouse interleukin 2 (IL-2) gene, Shiva la (a synthetic cecropinclass lytic peptide), and the SV40 polyadenylation/splice signal. A reverse-transcription PCR assay determined that two lines of transgenic mice were produced whose spleen-derived lymphocytes could be induced to transcribe and mature mRNA for Shiva la by exposure to 3.25 mg ml(-1) of Con A. Two lines were challenged with an inoculation of 5 X 10(4) Brucella abortus strain 2308. After four weeks, there were significantly fewer B. abortus organisms in the spleens of transgenic mice than in non-transgenic control mice of the same strain (p < 0.05). Since the controlling regions of the IL-2 enhancer and the amino acid sequence of the signal peptide are highly conservedamong several species, it is likely that this recombinant gene will function in other mammals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 11:08:29