Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
BASIC STUDIES ON THE APPLICATION OF AN ARTIFICIAL ESOPHAGUS USING CULTURED EPIDERMAL-CELLS
Autore:
NAGASHIMA A; ANDO N; SATO M; OZAWA S; KITAJIMA M;
Indirizzi:
KEIO UNIV,SCH MED,DEPT SURG,SHINJUKU KU,35 SHINANOMACHI TOKYO 160 JAPAN
Titolo Testata:
SURGERY TODAY-THE JAPANESE JOURNAL OF SURGERY
fascicolo: 10, volume: 27, anno: 1997,
pagine: 915 - 923
SICI:
0941-1291(1997)27:10<915:BSOTAO>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
KERATINOCYTES; DIFFERENTIATION; CULTIVATION; INVITRO; GROWTH;
Keywords:
ARTIFICIAL ESOPHAGUS; EPIDERMAL CELL CULTURE; GRANULATION TUBE; EPITHELIALIZATION; KERATINOCYTE GROWTH MEDIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
A. Nagashima et al., "BASIC STUDIES ON THE APPLICATION OF AN ARTIFICIAL ESOPHAGUS USING CULTURED EPIDERMAL-CELLS", SURGERY TODAY-THE JAPANESE JOURNAL OF SURGERY, 27(10), 1997, pp. 915-923

Abstract

In making an artificial esophagus, the transplantation of the epithelialized granulation tube fabricated by organized synthetic material was studied mainly from the viewpoint of preventing anastomotic leakage and struture formation. The possibility of epithelialization of the inner surface of a granulation tube using cultured epidermal cells was studied in rats and dogs. A stainless steel mesh tube coated with silicon served as the granulation tube. Epithelialization on the inner surface of a granulation tube was evaluated by seeding cultured epidermal cells. A skin sample was treated with dispase and trypsin to collect epidermal cells, which were cultured in a keratinocyte growth medium, Once confluence was achieved, the epidermal cell suspension was harvested using the following methods: trypsin treatment (n = 15), mechanicalseparation with a cell scraper (n = 6), and dispase treatment (n = 9). The cultured epidermal cell suspension was then seeded into the lumen of the granulation tubes, The attachment of cultured epidermal cellswas attained in 2 of 15 cases by trypsin treatment, and in 5 of 9 cases by dispase treatment. No attachment occurred using the cell scrapermethod. All attached epidermal cells exhibited a cobblestone appearance on the granulation tissue with a tendency toward stratification, These findings show that the inner surface of a steel mesh granulation tube was epithelialized by cultured epidermal cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 03:15:53