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Titolo:
DEVELOPMENTAL-CHANGES IN CALPAIN ACTIVITY, GLUR1 RECEPTORS AND IN THEEFFECT OF KAINIC ACID TREATMENT IN RAT-BRAIN
Autore:
BI X; CHEN J; BAUDRY M;
Indirizzi:
UNIV SO CALIF,NEUROSCI PROGRAM LOS ANGELES CA 90089
Titolo Testata:
Neuroscience
fascicolo: 4, volume: 81, anno: 1997,
pagine: 1123 - 1135
SICI:
0306-4522(1997)81:4<1123:DICAGR>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; ENTORHINAL CORTEX PRODUCE; INDUCED SEIZURE ACTIVITY; DAMAGE SYNDROME; DENTATE GYRUS; MEDIATED DEGRADATION; SPECTRIN DEGRADATION; PYRAMIDAL NEURONS; AMPA RECEPTORS; AREA CA1;
Keywords:
EPILEPSY; PLASTICITY; DEVELOPMENT; HIPPOCAMPUS; AMPA; SPECTRIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
X. Bi et al., "DEVELOPMENTAL-CHANGES IN CALPAIN ACTIVITY, GLUR1 RECEPTORS AND IN THEEFFECT OF KAINIC ACID TREATMENT IN RAT-BRAIN", Neuroscience, 81(4), 1997, pp. 1123-1135

Abstract

The cellular distribution of calpain activation and glutamate receptor 1 (GluR1) subunits of lpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors and their alterations following kainic acid-induced seizure were evaluated during postnatal development using antibodiesspecific for spectrin breakdown product and the C-terminus of GluR1 subunits. In the first postnatal week, most brain regions exhibited high levels of calpain activity that progressively decreased during the following weeks. The highest levels of spectrin breakdown product immunoreactivity were observed in the somata and proximal dendrites of hippocampal pyramidal cells, non-pyramidal neurons in stratum oriens, and cortical neurons. In general, during the first two postnatal weeks, kainic acid treatment induced a decrease in spectrin breakdown product immunoreactivity in neuronal cell bodies and an increase in dendritic fields. Obvious elevation in spectrin breakdown product immunoreactivity in selective non-pyramidal cells in stratum oriens started at postnatal day 14, and was further evidenced by postnatal day 21. Likewise, massive calpain activation in subpopulations of neurons in some thalamic nuclei, amygdala, and pyriform cortex was observed after the third postnatal week. GluR1 subunits were highly expressed throughout the forebrain in the first postnatal week, further increased during the second postnatal week, decreased thereafter, and reached adult levels afterpostnatal day 21. In cortex, intense GluR1 immunostaining was found in the somata and proximal processes of pyramidal and non-pyramidal neurons, with the non-pyramidal neurons in layers IV through VI exhibiting the densest immunolabelling. In the first two postnatal weeks, the somata of hippocampal pyramidal neurons exhibited intense GluR1 immunostaining that became more dendritic in the subsequent developmental period. While hilar cells exhibited a similar developmental pattern as CAregions, the molecular layer of dentate gyrus exhibited weak immunoreactivity from postnatal day 7 to postnatal day 14. The early increase in GluR1 immunoreactivity in hippocampal pyramidal layer following kainic acid treatment occurred throughout the developmental period, whilethe later decrease in CA regions, amygdala, and pyriform cortex was observed only in postnatal day 21 animals. The combined immunocytochemical studies of spectrin breakdown product localization and GluR1 expression indicate that calpain activation might play an important role insynaptic formation, developmental regulation of synaptic plasticity, and neuronal vulnerability to excitotoxicity during postnatal development. Moreover, calpain-mediated modulation of lpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors might underlie these processes. (C) 1997 IBRO. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 03:25:53