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Titolo:
EFFECT OF METABOLIC-ACIDOSIS ON THE POTASSIUM CONTENT OF BONE
Autore:
BUSHINSKY DA; GAVRILOV K; CHABALA JM; FEATHERSTONE JDB; LEVISETTI R;
Indirizzi:
UNIV ROCHESTER,SCH MED,DEPT MED,NEPHROL UNIT,601 ELMWOOD AVE,BOX 675 ROCHESTER NY 14642 STRONG MEM HOSP,NEPHROL UNIT ROCHESTER NY 14642 UNIV CHICAGO,DEPT PHYS,ENRICO FERMI INST CHICAGO IL 60637 UNIV CALIF SAN FRANCISCO,DEPT RESTORAT DENT SAN FRANCISCO CA 94143
Titolo Testata:
Journal of bone and mineral research
fascicolo: 10, volume: 12, anno: 1997,
pagine: 1664 - 1671
SICI:
0884-0431(1997)12:10<1664:EOMOTP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SURFACE ION COMPOSITION; URINARY CALCIUM EXCRETION; CARBONATED APATITES; IMAGING MICROANALYSIS; DISSOLUTION BEHAVIOR; SYNTHETIC APATITES; MOUSE CALVARIAE; INVITRO; MICROPROBE; RESOLUTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
D.A. Bushinsky et al., "EFFECT OF METABOLIC-ACIDOSIS ON THE POTASSIUM CONTENT OF BONE", Journal of bone and mineral research, 12(10), 1997, pp. 1664-1671

Abstract

Metabolic acidosis induces resorption of cultured bone, resulting in a net efflux of calcium (Ca) from the bone and an apparent loss of mineral potassium (K), However, in these organ cultures, there is diffusion of K between the medium and the crystal lattice, causing difficultyin interpretation of the acid-induced changes in mineral ion composition, To determine the effects of acidosis on bone mineral K, we injected 4-day-old neonatal mice with pure stable isotope K-41, equal to similar to 5% of their total body K. Calvariae were dissected 24 h later and then cultured for 24 h in medium without added K-41, either at pH similar to 7.4 (Ctl) or at pH similar to 7.1 (Ac), with or without theosteoclastic inhibitor calcitonin (3 x 10(-9) M, CT), The bone isotopic ion content was determined with a high-resolution scanning ion microprobe utilizing secondary ion mass spectrometry, K-41 is present in nature at 6.7% of total K, The injected K-41 raised the ratio of bone K-41/(K-39 + K-41) to 9.8 +/- 0.5% On the surface (ratios of counts persecond of detected secondary ions, mean +/- 95% confidence interval) but did not alter the ratio in the interior (6.9 +/- 0.4%), indicatingbiological incorporation of the K-41 into the mineral surface, The ratios of K-41/Ca-40 on the surface of Ctl calvariae was 14.4 +/- 1.2, indicating that bone mineral surface is rich in K compared with Ca, Compared with Ctl, Ac caused a marked increase in the net Ca efflux from bone that was blocked by CT, Ac also induced a marked fall in the ratio of K-41/Ca-40 on the surface of the calvariae (4.3 +/- 0.5, p < 0.01VS, Ctl), which was partially blocked by CT (8.2 +/- 0.9, p < 0.01 vs, Ctl and vs, Ac), indicating that Ac causes a greater release of bonemineral K than Ca which is partially blocked by CT, Thus, bone mineral surface is rich in K relative to Ca, acidosis induces a greater release of surface mineral K than Ca, and osteoclastic function is necessary to support the enriched levels of surface mineral K in the presenceof acidosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 20:18:59