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Titolo:
IDENTIFICATION OF MOLECULAR MARKERS LINKED TO THE YR15 STRIPE RUST RESISTANCE GENE OF WHEAT ORIGINATED IN WILD EMMER WHEAT, TRITICUM-DICOCCOIDES
Autore:
SUN GL; FAHIMA T; KOROL AB; TURPEINEN T; GRAMA A; RONIN YI; NEVO E;
Indirizzi:
UNIV HAIFA,INST EVOLUT IL-31905 HAIFA ISRAEL UNIV HAIFA,INST EVOLUT IL-31905 HAIFA ISRAEL AGR RES ORG,VOLCANI CTR IL-50250 BET DAGAN ISRAEL
Titolo Testata:
Theoretical and Applied Genetics
fascicolo: 4, volume: 95, anno: 1997,
pagine: 622 - 628
SICI:
0040-5752(1997)95:4<622:IOMMLT>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
POWDERY MILDEW RESISTANCE; BULKED SEGREGANT ANALYSIS; NEAR-ISOGENIC LINES; RFLP MARKERS; PUCCINIA-STRIIFORMIS; RAPID METHOD; LOCUS; BARLEY; DNA; TOMATO;
Keywords:
GENE MAPPING; RAPD; RFLP; STRIPE RUST; TRITICUM DICOCCOIDES; TRITICUM DURUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
G.L. Sun et al., "IDENTIFICATION OF MOLECULAR MARKERS LINKED TO THE YR15 STRIPE RUST RESISTANCE GENE OF WHEAT ORIGINATED IN WILD EMMER WHEAT, TRITICUM-DICOCCOIDES", Theoretical and Applied Genetics, 95(4), 1997, pp. 622-628

Abstract

The Yr15 gene of wheat confers resistance to the stripe rust pathogenPuccinia striiformis West., which is one of the most devastating diseases of wheat throughout the world. In the present study, molecular markers flanking the Yr15 gene of wheat have been identified using the near-isogenic-lines approach. RFLP screening of 76 probe-enzyme combinations revealed one polymorphic marker (Nor/TaqI) between the susceptible and the resistant lines. In addition, out of 340 RAPD primers tested, six produced polymorphic RAPD bands between the susceptible and theresistant lines. The genetic linkage of the polymorphic markers was tested on segregating F-2 population (123 plants) derived from crosses between stripe rust-susceptible Triticum durum wheat, cv D447, and a BC3F9 resistant line carrying Yr15 in a D447 background. A 2.8-kb fragment produced by the Nor RFLP probe and a 1420-bp PCR product generatedby the RAPD primer OPB13 showed linkage, in coupling, with the Yr15 gene. Employing the standard maximum-likelihood technique it was found that the order OPB13(1420)- Yr15-Nor1 on chromosome 1B appeared to be no less than 1000-times more probable than the closest alternative, The map distances between OPB13(1420)-Yr15-Nor1 are 27.1 cM and 11.0 cM for-the first and second intervals, respectively. The application of marker-assisted selection for the breeding of new wheat cultivars with the stripe rust resistance gene is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:18:37