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Titolo:
CRITICAL ROLE OF LIPID-COMPOSITION IN MEMBRANE PERMEABILIZATION BY RABBIT NEUTROPHIL DEFENSINS
Autore:
HRISTOVA K; SELSTED ME; WHITE SH;
Indirizzi:
UNIV CALIF IRVINE,DEPT PHYSIOL & BIOPHYS IRVINE CA 92697 UNIV CALIF IRVINE,DEPT PHYSIOL & BIOPHYS IRVINE CA 92697 UNIV CALIF IRVINE,DEPT PATHOL IRVINE CA 92697
Titolo Testata:
The Journal of biological chemistry
fascicolo: 39, volume: 272, anno: 1997,
pagine: 24224 - 24233
SICI:
0021-9258(1997)272:39<24224:CROLIM>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
VESICLE CONTENTS; ANTIMICROBIAL PEPTIDES; GRANULOCYTE PEPTIDES; CANDIDA-ALBICANS; FUSION; BILAYERS; MECHANISM; PHOSPHATIDYLETHANOLAMINE; EXTRUSION; KINETICS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
K. Hristova et al., "CRITICAL ROLE OF LIPID-COMPOSITION IN MEMBRANE PERMEABILIZATION BY RABBIT NEUTROPHIL DEFENSINS", The Journal of biological chemistry, 272(39), 1997, pp. 24224-24233

Abstract

We have examined the interactions of the six known rabbit neutrophil defensin antimicrobial peptides with large unilamellar vesicles (LW) made from various lipid mixtures based an the lipid composition of Escherichia coli membranes. We find that the permeabilization of LUV made from E. coli whole lipid extracts differs dramatically from that of single-component LUV made from palmitoyl-oleoyl-phosphatidylglcerol (POPG). Specifically, defensins NP-1, NP-2, NP-3A, NP-3B, and a natural mixture of the six defensins cause fast nonpreferential leakage of high molecular weight dextrans as well as the low molecular weight fluorophore/ quencher pair 8-aminonapthalene-1,3,6 trisulfonic acid (ANTS)/p-xylene-bis-pyridium bromide (DPX) from E. coli whole lipid LUV through large, transient membrane lesions. In contrast, release of ANTS/DPX form POPG LUV induced by the defensins is slow and graded with preference for DPX (Hristova, K., Selstad, M. E., and White, S. H. (1996) Biochemistty 35, 11888-11894). Interestingly, defensins NP-4 and NP-5 alonedo not induce leakage from E. Coli whole lipid LUV, whereas only NP-4is ineffective with POPG LW, Examination of She sequences of the six defensins suggests that the inactivity of NP-4 and NP-5 may be due to their lower net positive charge and/or the substitution of a Thr for the Arg or Lys that follows the fourth Cys residue. We found the presence of three major lipid components of E. coli whole lipid to be essential for creation of the large lesions observed in LUV: phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin, Cardiolipin appears to play a key role because no leakage can be induced when only phosphatidylglycerol, and phosphatidylethanolamine are present. These resultsindicate the importance of membrane lipid composition in the permeabilization of cell membranes by rabbit defensins.

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Documento generato il 06/12/20 alle ore 00:04:45