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Titolo:
NITRIC-OXIDE SYNTHASE (NOS)-I DURING POSTNATAL-DEVELOPMENT IN RAT ANDMOUSE SKELETAL-MUSCLE
Autore:
CHRISTOVA T; GROZDANOVIC Z; GOSSRAU R;
Indirizzi:
FREE UNIV BERLIN,UNIV CLIN BENJAMIN FRANKLIN,DEPT MOL ANAT & CELL BIOL,INST ANAT D-14195 BERLIN GERMANY FREE UNIV BERLIN,UNIV CLIN BENJAMIN FRANKLIN,DEPT MOL ANAT & CELL BIOL,INST ANAT D-14195 BERLIN GERMANY MED UNIV SOFIA,INST ANAT & HISTOL BG-1431 SOFIA BULGARIA
Titolo Testata:
Acta histochemica
fascicolo: 3, volume: 99, anno: 1997,
pagine: 311 - 324
SICI:
0065-1281(1997)99:3<311:NS(DPI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
DUCHENNE MUSCULAR-DYSTROPHY; SARCOLEMMA; HISTOCHEMISTRY; EXPRESSION; DEFICIENT; FIBERS; CELLS;
Keywords:
NITRIC OXIDE SYNTHASE I; NOS I; SKELETAL MUSCLE; NEUROMUSCULAR JUNCTIONS; SARCOLEMMA; DYSTROPHIN COMPLEX; DEVELOPMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
T. Christova et al., "NITRIC-OXIDE SYNTHASE (NOS)-I DURING POSTNATAL-DEVELOPMENT IN RAT ANDMOUSE SKELETAL-MUSCLE", Acta histochemica, 99(3), 1997, pp. 311-324

Abstract

Previous studies on adult rat and mouse skeletal muscles have shown the spatial association of nitric oxide synthase (NOS) I to the dystrophin complex (DC) in the sarcolemma of type II fibers and, in combination with the NMDA receptor-1 (NMDAR-1), an accumulation of the enzyme at the neuromuscular junctions (NMJ) of this fiber type. Using immunohistochemistry, enzyme histochemistry and alpha-bungarotoxin labeling wereport here temporal relationships of NOS I, members of the DC, othercomponents of the cortical cytoskeleton in the junctional and non-junctional sarcolemma as well as of molecules involved in NMJ transmission of either type I or II myofibers especially in head and neck musclesduring postnatal rat and mouse development. Fiber typing was performed by specific anti-myosin antibodies. Beginning with postnatal day (PD) 1 in both fiber types dystrophin, dystrophin-associated glycoproteins (DAG), beta-dystroglycan, alpha-sarcoglycan (adhalin) and spectrin were present in; the junctional and extrajunctional sarcolemma, while utrophin, acetylcholinesterase, alpha-bungarotoxin labeled acetylcholine receptors were concentrated in the NMJ of both fiber types. NOS I activity and immunoreactivity were only found in the NMJ region of type II fibers, where NMDAR-1 appeared around PD 15. Primarily in the tongue there was no strict correlation between muscle fiber type and NOS I behaviour during early postnatal development, and muscle fibers not reactive for myosin antibodies against both fiber types were negative orpositive for NOS I but always positive for the other molecules eitherin both the junctional and extrajunctional sarcolemma or in the NMJ only; later all muscle fibers of the tongue were of type II and NOS I-positive. Maturation of enzyme activities, immunoreactivities and AChR intensity depended on the respective muscle and can last until PD 50; in the tongue and neck muscles they appeared to increase approximatelyuntil PD 20 or 25. In conclusion, in type II fibers of rat and mouse skeletal muscle all molecules with the exception of NMDAR-1 and relevant for NOS I targeting and positioning as well as function inside and outside the NMJ are already present at birth, but their concentrationsand/or activities increase postnatally, and the adult situation appears to be reached between the third and seventh week of postnatal life. Therefore, initial interactions between NOS I and the other moleculesnecessary for the formation of the NOS I-DC in and on the way to the sarcolemma presumably take place before birth.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 04:35:23