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Titolo:
INCREASED MUTABILITY BY OXIDATIVE STRESS IN OXYR-DEFICIENT ESCHERICHIA-COLI AND SALMONELLA-TYPHIMURIUM CELLS - CLONAL OCCURRENCE OF THE MUTANTS DURING GROWTH ON NONSELECTIVE MEDIA
Autore:
BLANCO M; HERRERA G; URIOS A;
Indirizzi:
FDN VALENCIANA INVEST BIOMED,INST INVEST CITOL,AMADEO SABOYA 4 E-46010 VALENCIA SPAIN
Titolo Testata:
Mutation research. Mutation research letters
fascicolo: 4, volume: 346, anno: 1995,
pagine: 215 - 220
SICI:
0165-7992(1995)346:4<215:IMBOSI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPONTANEOUS MUTAGENESIS; DNA DAMAGE; PROTEINS; CLONING; OXYGEN; RESISTANCE; PEROXIDE; REGULON; GENES; MUTY;
Keywords:
OXIDATIVE STRESS; OXYR(-) MUTATION; PREADAPTIVE SOS MUTAGENESIS; T-BUTYL HYDROPEROXIDE;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
M. Blanco et al., "INCREASED MUTABILITY BY OXIDATIVE STRESS IN OXYR-DEFICIENT ESCHERICHIA-COLI AND SALMONELLA-TYPHIMURIUM CELLS - CLONAL OCCURRENCE OF THE MUTANTS DURING GROWTH ON NONSELECTIVE MEDIA", Mutation research. Mutation research letters, 346(4), 1995, pp. 215-220

Abstract

Escherichia coli and Salmonella typhimurium strains deficient in the OxyR-regulated adaptive response to oxidative stress were used to study the mode in which spontaneous SOS-dependent mutations are generated in a distressed bacterial population. When assayed on supplemented selective medium, the E. coli strain IC3821 (trpE65), carrying the Delta oxyR30 mutation and containing the plasmid pRW144 (mucA/B), showed a frequency of spontaneous Trp(+) revertants similar to that of the oxyR() control. Instead, the IC3821 strain exhibited an enhancement in theclonal occurrence of spontaneous revertants arising at random during growth on a nonselective medium. A similar enhancement was observed for the S. typhimurium strain TA4125 (hisG428 Delta oxyR2). The mutator effect observed in oxyR(-) cells would be induced by an increased background of reactive oxygen species; it provides a model for studying the mutability of a cell population constantly exposed to mutation-inducing agents. In the IC3821 strain, revertants were induced by t-butyl hydroperoxide with higher efficiency than in oxyR(+). We suggest that strain IC3821 could be useful for the detection of SOS-dependent mutagenesis induced by chemical oxidants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 02:31:37