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Titolo:
SNF2-BETA-BRG1 IS ESSENTIAL FOR THE VIABILITY OF F9 MURINE EMBRYONAL CARCINOMA-CELLS
Autore:
SUMIICHINOSE C; ICHINOSE H; METZGER D; CHAMBON P;
Indirizzi:
CU STRASBOURG,CNRS INSERM ULP,INST GENET & BIOL MOL & CELLULAIRE,COLLFRANCE,BP 163 F-67404 ILLKIRCH GRAFFENS FRANCE CU STRASBOURG,CNRS INSERM ULP,INST GENET & BIOL MOL & CELLULAIRE,COLLFRANCE F-67404 ILLKIRCH GRAFFENS FRANCE
Titolo Testata:
Molecular and cellular biology
fascicolo: 10, volume: 17, anno: 1997,
pagine: 5976 - 5986
SICI:
0270-7306(1997)17:10<5976:SIEFTV>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
YEAST SWI/SNF COMPLEX; RETINOIC ACID RECEPTOR; NUCLEOSOME REMODELING FACTOR; TERATOCARCINOMA STEM-CELLS; TRANSCRIPTIONAL ACTIVATORS; SACCHAROMYCES-CEREVISIAE; GLUCOCORTICOID RECEPTOR; RETINOBLASTOMA PROTEIN; ESTROGEN-RECEPTOR; DROSOPHILA-BRAHMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
C. Sumiichinose et al., "SNF2-BETA-BRG1 IS ESSENTIAL FOR THE VIABILITY OF F9 MURINE EMBRYONAL CARCINOMA-CELLS", Molecular and cellular biology, 17(10), 1997, pp. 5976-5986

Abstract

The yeast and animal SNF-SWI and related multiprotein complexes are thought to play an important role in processes, such as transcription factor binding to regulatory elements, which require nucleosome remodeling in order to relieve the repressing effect of packaging DNA in chromatin, There are two mammalian homologs of the yeast SNF2-SW12 subunitprotein, SNF2 alpha-brm and SNF2 beta-BRG1, and overexpression of either one of them has been shown to enhance transcriptional activation by glucocorticoid. estrogen, and retinoic acid (RA) receptors in transiently transfected cells, We have investigated here the function of SNF2 beta-BRG1 in the RA receptor-retinoid X receptor-mediated transduction of the retinoid signal in F9 embryonal carcinoma (EC) cells which differentiate into endodermal-like cells upon RA treatment. The two SNF2 beta-BRG1 alleles have been targeted by homologous recombination andsubsequently disrupted by using a conditional Cre recombinase. We show that F9 EC cells inactivated on both SNF2 beta alleles are not viable and that heterozygous mutant cells are affected in proliferation butnot in RA-induced differentiation. Thus, in F9 EC cells, SNF2 beta-BRG1 appears to play an essential role in basal processes involved in cell proliferation, in addition to its putative role in the activation of transcription mediated by nuclear receptors.

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Documento generato il 23/01/21 alle ore 03:34:43