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Titolo:
NONINVASIVE MEASUREMENT OF AIRWAY RESPONSIVENESS IN ALLERGIC MICE USING BAROMETRIC PLETHYSMOGRAPHY
Autore:
HAMELMANN E; SCHWARZE J; TAKEDA K; OSHIBA A; LARSEN GL; IRVIN CG; GELFAND EW;
Indirizzi:
NATL JEWISH CTR IMMUNOL & RESP MED,DEPT PEDIAT,DIV BASIC SCI,1400 JACKSON ST DENVER CO 80206 NATL JEWISH CTR IMMUNOL & RESP MED,DEPT PEDIAT,DIV BASIC SCI DENVER CO 80206 NATL JEWISH CTR IMMUNOL & RESP MED,DEPT MED DENVER CO 80206 NATL JEWISH CTR IMMUNOL & RESP MED,DEPT PEDIAT,DIV PULM MED DENVER CO80206
Titolo Testata:
American journal of respiratory and critical care medicine
fascicolo: 3, volume: 156, anno: 1997,
pagine: 766 - 775
SICI:
1073-449X(1997)156:3<766:NMOARI>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIGS; PASSIVE TRANSFER; MURINE MODEL; ASTHMA; HYPERREACTIVITY; HYPERRESPONSIVENESS; INTERLEUKIN-5; ANTIGEN; MOUSE; SENSITIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
E. Hamelmann et al., "NONINVASIVE MEASUREMENT OF AIRWAY RESPONSIVENESS IN ALLERGIC MICE USING BAROMETRIC PLETHYSMOGRAPHY", American journal of respiratory and critical care medicine, 156(3), 1997, pp. 766-775

Abstract

To study the mechanisms and kinetics underlying the development of increased airway responsiveness (AR) after allergic sensitization, animal models have been invaluable. Using barometric wholebody plethysmography and increases in enhanced pause (Penh) as an index of airway obstruction, we measured responses to inhaled methacholine in conscious, unrestrained mice after sensitization and airway challenge with ovalbumin (OVA). Sensitized and challenged animals had significantly increasedAR to aerosolized methacholine compared with control animals. AR measured as Penh was associated with increased IgE production and eosinophil lung infiltration. In a separate approach we confirmed the involvement of the lower airways in the response to aerosolized methacholine using tracheotomized mice. Increases in Penh values after methacholine challenge were also correlated with increased intrapleural pressure, measured via an esophageal tube. Lastly, mice demonstrating AR using a noninvasive technique also demonstrated increased pulmonary resistanceresponses to aerosolized methacholine when measured using an invasivetechnique the following day in the same animals. The increases in Penh values were inhibited by pretreatment of the mice with a beta(2)-agonist. These data indicate that measurement of AR to inhaled methacholine by barometric whole-body plethysmography is a valid indicator of airway hyperresponsiveness after allergic sensitization in mice. The measurement of AR in unrestrained, conscious animals provides new opportunities to evaluate the mechanisms and kinetics underlying the development and maintenance of airway hyperresponsiveness and to assess various therapeutic interventions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 00:50:36