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Titolo:
DETERMINATION OF INDOMETHACIN AND MEFENAMIC-ACID IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
Autore:
NIOPAS I; MAMZORIDI K;
Indirizzi:
ARISTOTELIAN UNIV THESSALONIKI,DEPT PHARM GR-54006 THESSALONIKI GREECE ARISTOTELIAN UNIV THESSALONIKI,ACHEPA HOSP,DEPT MED,PAEDIAT CLIN 4 GR-54006 THESSALONIKI GREECE
Titolo Testata:
Journal of chromatography B. Biomedical applications
fascicolo: 2, volume: 656, anno: 1994,
pagine: 447 - 450
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTI-INFLAMMATORY DRUGS; DUCTUS-ARTERIOSUS; URINE; CARTRIDGES; EXTRACTION; CLOSURE; INFANTS; BLOOD; SERUM; HPLC;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
I. Niopas e K. Mamzoridi, "DETERMINATION OF INDOMETHACIN AND MEFENAMIC-ACID IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY", Journal of chromatography B. Biomedical applications, 656(2), 1994, pp. 447-450

Abstract

Indomethacin and mefenamic acid are widely used clinically as non-steroidal anti-inflammatory agents. Both drugs have also been found effective to produce closure of patent ductus arteriosus in premature neonates. A simple, rapid, sensitive and reliable HPLC method is described for the determination of indomethacin and mefenamic acid in human plasma. As these drugs are not applied together, the compounds are alternately used as analyte and internal standard. Plasma was deproteinized with acetonitrile, the supernatant fraction was evaporated to dryness and the resulting residue was reconstituted in the mobile phase and injected into the HPLC system. The chromatographic separation was performed on a C-18 column (250 x 4.6 mm I.D.) using 10 mM phosphoric acid-acetonitrile (40:60, v/v) as the mobile phase and both drugs were detected at 280 nm. The calibration graphs were linear with a correlation coefficient (r) of 0.999 or better from 0.1 to 10 mu g/ml and the detection limits were 0.06 mu g/ml for indomethacin and 0.08 mu g/ml for mefenamic acid, for 50-mu l plasma samples. The method was not interferedwith by other plasma components and has been found particularly useful for paediatric use. The within-day precision and accuracy of the method were evaluated for three concentrations in spiked plasma samples. The coefficients of variation were less than 5% and the accuracy was nearly 100% for both drugs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 16:14:58