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Title
Optimization of culture conditions of an Argentine strain of Dunaliella salina (Chlorophyta), for the synthesis of beta-carotene
Author:
Miravalles, AB; Leonardi, PI;
Addresses:
Univ Nacl Sur, Dept Biol Bioquim & Farm, Lab Plantas Avasculares, RA-8000 Bahia Blanca, Buenos Aires, Argentina Univ Nacl Sur Bahia Blanca Buenos Aires Argentina RA-8000 ires, Argentina
Heading Title:
WOCMAP-2: SECOND WORLD CONGRESS ON MEDICINAL AND AROMATIC PLANTS FOR HUMANWELFARE, PROCEEDINGS
issue: 502, , year: 1999,
pages: 153 - 157
SICI:
0567-7572(1999):502<153:OOCCOA>2.0.ZU;2-J
Source:
ISI
Language:
ENG
Subject:
POLYBLEPHARIDACEAE; TEODORESCO; ALGAE; MEDIA;
Keywords:
alga; carotenogenesis; Chlorophyceae; growth; microalga;
Document Type:
Article
Nature:
Book in Series
Edition:
Agriculture,Biology & Environmental Sciences
Citations:
14
Reviewed:
Addresses for extracts:
Address: Miravalles, AB Univ Nacl Sur, Dept Biol Bioquim & Farm, Lab Plantas Avasculares, RA-8000 Bahia Blanca, Buenos Aires, Argentina Univ Nacl Sur Bahia Blanca Buenos Aires Argentina RA-8000
Citation:
A.B. Miravalles and P.I. Leonardi, "Optimization of culture conditions of an Argentine strain of Dunaliella salina (Chlorophyta), for the synthesis of beta-carotene", ACTA HORT, (502), 1999, pp. 153-157

Abstract

Dunaliella salina is a green microalga, which synthesises and stores largeamounts of beta-carotene under certain environmental conditions. For this reason it is grown massively for commercial purposes in several countries. The aim of this study was to find the optimal conditions for growth in laboratory of an Argentine strain of D. salina, for the synthesis of beta-carotene. The samples were isolated and grown in two culture media: modified Johnson and ASP-H. Four concentrations of ClNa (0M, 1.5M, 3M and 4.5M) were used with a pH of 7-7.5, at 30-36 degrees C and continuous light, under a photon flux of 290 mu E m(-2) s(-1). Cell density was obtained by successive counts in a Newbahuer chamber. Growth rate(K) and doubling-time (Td) during the exponential were determined in each case. The cultures of the low concentrations presented an increase in cell density with a greater differentiation of phases than the cultures of high concentrations. D. salina toleratedwide concentration ranges of ClNa under laboratory conditions, synthesizing beta-carotene in concentrations of 4.5M and 3M, one month and three months after the culture was started, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Bologna University, Review Catalogue
Document created on 15/07/19 at 20:00:14