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Title
A MOLECULAR MECHANISM OF INTEGRIN REGULATION FROM BONE-CELLS STIMULATED BY ORTHODONTIC FORCES
Author:
CARVALHO RS; BUMANN A; SCHWARZER C; SCOTT E; YEN EHK;
Addresses:
DEPT ORTHOPED SURG,LAB TREATMENT SKELETAL DISORDERS,11TH FLOOR ENDERSBLDG,320 LONGWOOD AVE BOSTON MA 02115 UNIV MANITOBA,DEPT PREVENT DENT SCI WINNIPEG MB R3T 2N2 CANADA CHRISTIAN ALBRECHTS UNIV KIEL,DEPT ORTHODONT KIEL GERMANY UNIV MANITOBA,DEPT ANAT WINNIPEG MB R3T 2N2 CANADA UNIV MANITOBA,DEPT ORAL BIOL WINNIPEG MB R3T 2N2 CANADA UNIV BRITISH COLUMBIA,FAC DENT VANCOUVER BC V5Z 1M9 CANADA
Heading Title:
European journal of orthodontics
issue: 3, volume: 18, year: 1996,
pages: 227 - 235
SICI:
0141-5387(1996)18:3<227:AMMOIR>2.0.ZU;2-N
Source:
ISI
Language:
ENG
Subject:
MOUSE INTERPARIETAL SUTURE; TOOTH MOVEMENT; GENE-EXPRESSION; FIBRONECTIN; ADHESION; COLLAGEN; BINDING; TISSUE; RECEPTOR; SUBUNIT;
Document Type:
Article
Nature:
Serial
Edition:
Science Citation Index Expanded
Citations:
44
Reviewed:
Addresses for extracts:
Citation:
R.S. Carvalho and others, "A MOLECULAR MECHANISM OF INTEGRIN REGULATION FROM BONE-CELLS STIMULATED BY ORTHODONTIC FORCES", European journal of orthodontics, 18(3), 1996, pp. 227-235

Abstract

The purpose of this paper is to discuss a molecular mechanism in the signal transduction pathways of the regulation of integrin genes taking place in bone cells as a result of orthodontic or mechanical stimulation. Human osteosarcoma (HOS) TE-85 cells were cultured in Dulbecco'smodified Eagle's medium (DMEM)/F-12 and grown to confluency in Flexercell type I dishes and orthodontic forces were applied to the cells via an intermittent strain of 3 cycles/minute using the Flexercell Strain Unit System for periods of 15 and 30 minutes, 2 and 24 hours and 3 days. Antibodies against beta(l) and alpha(v) integrins were immunolocalized in strained and unstrained cultures. Total RNA was extracted andcDNA probes were used to measure at various mRNA expression of beta(1) (1.2 kb) and alpha(v) (1.1 kb) integrins. A cDNA probe for cyclophylin (750 b) was used for controls of gene expression. Results showed that mechanical stimulation caused a reorganization of integrin distribution in comparison with non-stimulated controls. mRNA for beta(l) expression showed a marked increase at 30 minutes and 3 days, while mRNA levels for alpha(v) did not change with strain. The selective expression of integrins mRNA is indicative of a specific gene regulation by mechanical stimulation in the cells studied.

ASDD Area Sistemi Dipartimentali e Documentali, Bologna University, Review Catalogue
Document created on 23/01/19 at 19:23:30