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Titolo:
Different expression patterns for ubiquitous calpains and Capn3 splice variants in monkey ocular tissues
Autore:
Nakajima, T; Fukiage, C; Azuma, M; Ma, H; Shearer, TR;
Indirizzi:
Senju Pharmaceut Co Ltd, Res Lab, Nishi Ku, Kobe, Hyogo 6512241, Japan Senju Pharmaceut Co Ltd Kobe Hyogo Japan 6512241 be, Hyogo 6512241, Japan Oregon Hlth Sci Univ, Dept Oral Mol Biol & Ophthalmol, Portland, OR 97201 USA Oregon Hlth Sci Univ Portland OR USA 97201 halmol, Portland, OR 97201 USA
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
fascicolo: 1-2, volume: 1519, anno: 2001,
pagine: 55 - 64
SICI:
0167-4781(20010528)1519:1-2<55:DEPFUC>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUSCLE-SPECIFIC CALPAIN; RAT LENS; PHYSIOLOGICAL-FUNCTION; MOLECULAR-CLONING; SKELETAL-MUSCLE; MESSENGER-RNA; LP82 CALPAIN; P94; PURIFICATION; IDENTIFICATION;
Keywords:
proteinase; calpain; rat; monkey; calpain 3 isoform;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Azuma, M Senju Pharmaceut Co Ltd, Res Lab, Nishi Ku, 1-5-4 Murotani, Kobe,Hyogo 6512241, Japan Senju Pharmaceut Co Ltd 1-5-4 Murotani Kobe Hyogo Japan 6512241 n
Citazione:
T. Nakajima et al., "Different expression patterns for ubiquitous calpains and Capn3 splice variants in monkey ocular tissues", BBA-GENE ST, 1519(1-2), 2001, pp. 55-64

Abstract

The purpose of the present investigation was to compare the expression of ubiquitous and tissue-specific calpains in ocular tissues from the Macaca fascicularis monkey. Calpain isoforms in retina and corneal epithelium from adult M. fascicularis monkeys were characterized by RT-PCR, cDNA cloning and sequencing. Calpain isoform activities in ocular tissues were investigated by fractionation on DEAE-HPLC, immunoblotting, and casein zymography. Capn3 splice variants in the ocular tissues from rat, rabbit and monkey were compared after RT-PCR. RT-PCR analysis revealed that numerous splice variants of Capn3 were expressed in the epithelium from monkey cornea. The variants contained deletions or insertions in or around the IS1, IS2, and NS regions. The cDNAs for Capn3 variants were highly conserved, yet the expression patterns of the Capn3 isoforms were widely different among the mammalian species. In contrast, the expression patterns of ubiquitous calpains in ocular tissues were conserved among the mammalian species, and similarities between monkey and human cDNAs for Capn1 (mu -calpain) and Capn2 (m-calpain) were 98 and 99%, respectively. These results suggested that differences in expression patterns of Capn3 variants might be related to the function of each variant in a particular tissue or species. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/06/18 alle ore 17:01:36