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Titolo: Enantioseparation of S-carboxymethylcysteine and N-acetamidocarboxymethylcysteine by capillary electrophoresis using vancomycin
Autore: Fanali, S; Cartoni, C; Aturki, Z;
- Indirizzi:
- CNR, Ist Cromatog, Area Ric Roma, I-00016 Monterotondo, Roma, Italy CNR Monterotondo Roma Italy I-00016 ma, I-00016 Monterotondo, Roma, Italy
- Titolo Testata:
- JOURNAL OF SEPARATION SCIENCE
fascicolo: 9,
volume: 24,
anno: 2001,
pagine: 789 - 794
- SICI:
- 1615-9314(200109)24:9<789:EOSAN>2.0.ZU;2-4
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- ZONE-ELECTROPHORESIS; CHROMATOGRAPHIC-SEPARATION; CHIRAL SEPARATION; CYCLODEXTRINS; RESOLUTION; ENANTIOMERS; ACIDS; ANTIBIOTICS; DERIVATIVES; SELECTORS;
- Keywords:
- enantiomers; chiral; drugs; indirect UV detection; antibiotics; vancomycin;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Physical, Chemical & Earth Sciences
- Citazioni:
- 24
- Recensione:
- Indirizzi per estratti:
- Indirizzo: Fanali, S CNR, Ist Cromatog, Area Ric Roma, POB 10, I-00016 Monterotondo, Roma, Italy CNR POB 10 Monterotondo Roma Italy I-00016 erotondo, Roma, Italy
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- Citazione:
- S. Fanali et al., "Enantioseparation of S-carboxymethylcysteine and N-acetamidocarboxymethylcysteine by capillary electrophoresis using vancomycin", J SEP SCI, 24(9), 2001, pp. 789-794
Abstract
Racemic S-carboxymethylcysteine (DF-1794Y) and N-acetamido S-carboxymethylcysteine (DF-1796A) were resolved into their enantiomers by capillary electrophoresis using vancomycin as a chiral selector. Electrophoretic runs were carried out in a polyacrylamide-coated capillary filled with a backgroundelectrolyte composed of sorbic acid/histidine (pH = 4.5-6.5) and the appropriate concentration of vancomycin. The presence of the chiral selector (CS) in the detector path was avoided by using the partial filling-countercurrent method, where the CS filled only part of the capillary and was moving in the opposite direction to the analytes. Due to the relatively low absorption of the enantiomers studied, indirect UV detection was used in order to increase the sensitivity of the method. For method optimization the effect of several experimental parameters such as vancomycin concentration, bufferpH, and organic modifier type and concentration on enantioresolution and migration time were studied. The migration order was verified only for S-carboxymethylcysteine by injecting a mixture containing the racemic DF-1794Y spiked with the enantiomer S-carboxymethyl-L-cysteine. The L-isomer was found to migrate first.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/06/13 alle ore 12:24:01