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Titolo:
Partial recovery of light-independent chlorophyll biosynthesis in the chlL-deletion mutant of Synechocystis sp PCC 6803
Autore:
Wu, QY; Yu, JJ; Zhao, NM;
Indirizzi:
Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China Tsing Hua Univ Beijing Peoples R China 100084 ng 100084, Peoples R China
Titolo Testata:
IUBMB LIFE
fascicolo: 5, volume: 51, anno: 2001,
pagine: 289 - 293
SICI:
1521-6543(200105)51:5<289:PROLCB>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHLOROPLAST GENE; NITROGENASE;
Keywords:
chlorophyll biosynthesis; chlL gene; cyanobacterium; light-independent protochlorophyllide reductase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Wu, QY Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, PeoplesR China Tsing Hua Univ Beijing Peoples R China 100084 84, Peoples R China
Citazione:
Q.Y. Wu et al., "Partial recovery of light-independent chlorophyll biosynthesis in the chlL-deletion mutant of Synechocystis sp PCC 6803", IUBMB LIFE, 51(5), 2001, pp. 289-293

Abstract

A chlL-deletion mutant of Synechocystis sp. PCC 6803 designated as chlL(-)was unable to make significant amounts of chlorophyll in darkness. However, an apparent pseudorevertant has been generated spontaneously that can synthesize an increased amount of chlorophyll under light-activated heterotrophic growth conditions. Under these conditions, the chlorophyll content in this pseudorevertant was about 20% of that in the wild-type strain and about4 times more than that in the original and in the recently recreated chlL-deletion mutant. This is paralleled by increased performance of dark-grown cells in terms of chlorophyll fluorescence induction and oxygen evolution rates in the pseudorevertant versus in the original mutant. PCR analysis confirmed that the chlL(-) pseudorevertant mutant still lacked the chlL gene. These results imply that the light-independent chlorophyll biosynthesis pathway was partly recovered.

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Documento generato il 24/06/18 alle ore 17:04:29