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Titolo:
Phosphomannose isomerase: An efficient selectable marker for plant transformation
Autore:
Reed, J; Privalle, L; Powell, ML; Meghji, M; Dawson, J; Dunder, E; Suttie, J; Wenck, A; Launis, K; Kramer, C; Chang, YF; Hansen, G; Wright, M;
Indirizzi:
Novartis Agribusiness Biotechnol Res Inc, Res Triangle Pk, NC 27709 USA Novartis Agribusiness Biotechnol Res Inc Res Triangle Pk NC USA 27709 USA
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
fascicolo: 2, volume: 37, anno: 2001,
pagine: 127 - 132
SICI:
1054-5476(200103/04)37:2<127:PIAESM>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PSEUDOMONAS-AERUGINOSA; BIOSYNTHETIC-PATHWAY; NUCLEOTIDE-SEQUENCE; ESCHERICHIA-COLI; GENE MANA; PURIFICATION; MANNOSE; EXPRESSION; SELECTION; PMI;
Keywords:
mannose; biolistics; PMI; maize; wheat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Reed, J Novartis Agribusiness Biotechnol Res Inc, 3054 Cornwallis Rd, Res TrianglePk, NC 27709 USA Novartis Agribusiness Biotechnol Res Inc 3054 Cornwallis Rd Res Triangle Pk NC USA 27709
Citazione:
J. Reed et al., "Phosphomannose isomerase: An efficient selectable marker for plant transformation", IN VITRO-PL, 37(2), 2001, pp. 127-132

Abstract

Phosphomannose isomerase (PMI) catalyzes the reversible interconversion ofmannose 6-phosphate and fructose 6-phosphate. Plant cells lacking this enzyme are incapable of surviving on synthetic medium containing mannose as a carbon source. Maize, wheat and barley plants, genetically modified to express the Escherichia coli manA gene (Pmi) under the control of a plant promoter, were able to survive selection on mannose-containing medium. Transformation frequencies averaged 45% for maize transformation via Biolistics(TM),35% for maize Agrobacterium-mediated transformation, 20% for wheat, 3% forbarley, and 2% for watermelon transformation. Moreover, the frequencies exceeded those obtained for maize and wheat using the pat or bar gene with Basta(R) selection. A preliminary safety assessment has been conducted for PML Purified PMI protein demonstrates no adverse effects in an acute mouse toxicity test. Purified PMI protein was readily digested in simulated mammalian gastric and intestinal fluids. Plants derived from sugar beet and corn cells that had been genetically modified to express the E. coli manA gene were evaluated for biochemical changes in mannose-associated pathways. No detectable changes in glycoprotein profiles were detected in PMI-transformed plants as compared to nontransgenic controls. The yield and nutritional composition of grain from PMI-transformed com plants compared to their non-transformed isogenic counterparts were also determined and no statistically significant differences were found. The inherent safety of a system based on simple sugar metabolism coupled with high transformation frequencies for monocots make pmi an ideal selectable marker for plant transformation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/12/17 alle ore 23:31:01