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Titolo:
Cytological examination of rat amniotic epithelial cells and cell transplantation to the liver
Autore:
Nakajima, T; Enosawa, S; Mitani, T; Li, XK; Suzuki, S; Amemiya, H; Koiwai, O; Sakuragawa, N;
Indirizzi:
Natl Childrens Med Res Ctr, Setagaya Ku, Tokyo 1548509, Japan Natl Childrens Med Res Ctr Tokyo Japan 1548509 Ku, Tokyo 1548509, Japan Sci Univ Tokyo, Tokyo 162, Japan Sci Univ Tokyo Tokyo Japan 162Sci Univ Tokyo, Tokyo 162, Japan Natl Inst Neurosci, Tokyo, Japan Natl Inst Neurosci Tokyo JapanNatl Inst Neurosci, Tokyo, Japan
Titolo Testata:
CELL TRANSPLANTATION
fascicolo: 4-5, volume: 10, anno: 2001,
pagine: 423 - 427
SICI:
0963-6897(2001)10:4-5<423:CEORAE>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Keywords:
rat; amniotic cells; cell transplantation; gene carrier;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
8
Recensione:
Indirizzi per estratti:
Indirizzo: Enosawa, S Natl Childrens Med Res Ctr, Setagaya Ku, 3-35-31 Taishido, Tokyo 1548509, Japan Natl Childrens Med Res Ctr 3-35-31 Taishido Tokyo Japan 1548509
Citazione:
T. Nakajima et al., "Cytological examination of rat amniotic epithelial cells and cell transplantation to the liver", CELL TRANSP, 10(4-5), 2001, pp. 423-427

Abstract

It is hoped that amniotic epithelial cells can be useful in cell-mediated gene therapy. We report here an experimental cell transplantation model of amniotic cells in rats. There is an anatomical difference between human androdent embryos. We established a method to isolate amniotic cells that areequivalent to human amniotic epithelial cells. An amniotic membrane distinct from the yolk sac was carefully collected and teased in saline containing deoxyribonuclease and hyaluronidase, followed by collagenase digestion. The cell yield was approximately 10(6) cells per pregnant female (10(5) cells per fetus), roughly in proportion to the age of fetus used, and 60% of the isolated cells were attached to the dish under culture conditions. Telomerase activity was higher in the cells isolated from fetuses in the middle stage (day 13.5 to 15.5) than in the late stage (day 17.5 to 21.5). Adherentcells exhibited two to three times more cell division, resulting in a ninefold increase in the number of cells. Immunohistochemical analysis revealedthat approximately half of the adherent cells were albumin positive and formed clusters. The senescent cells survived for 2 months without apparent morphological changes. The adherent cells were able to be stored in liquid nitrogen and had a viability of 70% when thawed. Gene transduction with adenovirus vector was highly effective for rat amniotic cells, Transplantation of lacZ transfected amniotic cells into syngeneic rat liver resulted in theintegration of the transplanted cells in the liver structure and the cellssurvived for at least 30 days.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/06/18 alle ore 16:57:52