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Titolo:
Gene analysis of Vibrio cholerae NAGV14 pilus and its distribution
Autore:
Kuroki, H; Toma, C; Nakasone, N; Yamashiro, T; Iwanaga, M;
Indirizzi:
Univ Ryukyus, Fac Med, Dept Bacteriol, Okinawa 9030215, Japan Univ Ryukyus Okinawa Japan 9030215 ept Bacteriol, Okinawa 9030215, Japan Oita Med Univ, Dept Microbiol, Oita 8795593, Japan Oita Med Univ Oita Japan 8795593 iv, Dept Microbiol, Oita 8795593, Japan
Titolo Testata:
MICROBIOLOGY AND IMMUNOLOGY
fascicolo: 6, volume: 45, anno: 2001,
pagine: 417 - 424
SICI:
0385-5600(2001)45:6<417:GAOVCN>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MANNOSE-SENSITIVE HEMAGGLUTININ; TOXIN-COREGULATED PILI; EL-TOR; COLONIZATION FACTOR; FILAMENTOUS PHAGE; STRAINS; O139; IDENTIFICATION; PATHOGENESIS; PURIFICATION;
Keywords:
Vibrio cholerae non-O1/non-O139; type 4 pili; major pilin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Kuroki, H Univ Ryukyus, Fac Med, Dept Bacteriol, Okinawa 9030215, Japan Univ Ryukyus Okinawa Japan 9030215 iol, Okinawa 9030215, Japan
Citazione:
H. Kuroki et al., "Gene analysis of Vibrio cholerae NAGV14 pilus and its distribution", MICROB IMMU, 45(6), 2001, pp. 417-424

Abstract

Adhesive pilus of Vibrio cholerae O34, strain NAGV14, was genetically analyzed. The deduced amino acid (aa) sequence of the major pilin structural gene (VcfA) was 67% homologous to the MshA pilin in the N-terminal region, but no homology was found in the C-terminal region which contained the antigenic epitopes, Upstream and downstream flanking regions examined were highlyhomologous to mshB and mshC of the MSHA (mannose-sensitive hemagglutinin) gene locus. A short leader sequence and a pair of cysteines near the C-terminus which are the characteristics of type 4a pilus family were found. The major pilin structural gene of NAGV14 was compared to that of a strain V10 producing non-adhesive pill, The deduced aa sequences showed 60% homology, and the distance between two cysteines in the C-terminal region was different, A total of 177 V; cholerae strains were investigated for the presence of a type 4 pilus gene locus by PCR, and 95% were positive. The major pilin gene of NAGV14 was detected in 4 of 93 V. cholerae non-Ol, non-O139 strainstested, but none of the V. cholerae O1 and O139 (72 and 12 strains, respectively). Our result suggested that a type 4 pilus gene locus similar to theMSHA gene locus is widely distributed among T! choler ae strains. We proposed naming this type 4 pilus gene locus the VCF (for V. cholerae flexible pill) gene locus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/10/17 alle ore 11:10:26