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Titolo:
The class II phosphoinositide 3-kinase C2 alpha is activated by clathrin and regulates clathrin-mediated membrane trafficking
Autore:
Gaidarov, I; Smith, MEK; Domin, J; Keen, JH;
Indirizzi:
Thomas Jefferson Univ, Kimmel Canc Ctr, Philadelphia, PA 19107 USA Thomas Jefferson Univ Philadelphia PA USA 19107 hiladelphia, PA 19107 USA Univ London Imperial Coll Sci Technol & Med, Sch Med, London W12 0NN, England Univ London Imperial Coll Sci Technol & Med London England W12 0NN gland
Titolo Testata:
MOLECULAR CELL
fascicolo: 2, volume: 7, anno: 2001,
pagine: 443 - 449
SICI:
1097-2765(200102)7:2<443:TCIP3C>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANS-GOLGI NETWORK; MANNOSE 6-PHOSPHATE RECEPTOR; PLECKSTRIN HOMOLOGY DOMAIN; PHOSPHATIDYLINOSITOL 3-KINASE; ARRESTIN/CLATHRIN INTERACTION; ENDOCYTIC PATHWAY; COATED VESICLES; TERMINAL DOMAIN; BINDING DOMAIN; AP-2 COMPLEXES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Keen, JH Thomas Jefferson Univ, Kimmel Canc Ctr, Philadelphia, PA 19107 USA Thomas Jefferson Univ Philadelphia PA USA 19107 ia, PA 19107 USA
Citazione:
I. Gaidarov et al., "The class II phosphoinositide 3-kinase C2 alpha is activated by clathrin and regulates clathrin-mediated membrane trafficking", MOL CELL, 7(2), 2001, pp. 443-449

Abstract

Phosphoinositides play key regulatory roles in vesicular transport pathways in eukaryotic cells. Clathrin-mediated membrane trafficking has been shown to require phosphoinositides, but little is known about the enzyme(s) responsible for their formation. Here we report that clathrin functions as an adaptor for the class II PI 8-kinase C2 alpha (PI3K-C2 alpha), binding to its N-terminal region and stimulating its catalytic activity, especially toward phosphorylated inositide substrates. Further, we show that endogenous PI3K-C2 alpha is localized in coated pits and that exogenous expression affects clathrin-mediated endocytosis and sorting in the trans-Golgi network. These findings provide a mechanistic basis for localized inositide generation at sites of clathrin-coated bud formation, which, with recruitment of inositide binding proteins and subsequent synaptojanin-mediated phosphoinositide hydrolysis, may regulate coated vesicle formation and uncoating.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/12/17 alle ore 23:39:14