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Titolo:
Association between the level of ERCC-1 expression and the repair of cisplatin-induced DNA damage in human ovarian cancer cells
Autore:
Li, QD; Yu, JJ; Mu, CJ; Yunmbam, MK; Slavsky, D; Cross, CL; Bostick-Bruton, F; Reed, E;
Indirizzi:
NCI, Med Ovarian Canc Serv, Med Branch,Dev Therapeut Dept, Div Clin Sci,NIH, Bethesda, MD 20892 USA NCI Bethesda MD USA 20892 Dept, Div Clin Sci,NIH, Bethesda, MD 20892 USA
Titolo Testata:
ANTICANCER RESEARCH
fascicolo: 2A, volume: 20, anno: 2000,
pagine: 645 - 652
SICI:
0250-7005(200003/04)20:2A<645:ABTLOE>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE EXCISION-REPAIR; MESSENGER-RNA EXPRESSION; INTERSTRAND CROSS-LINKS; CARCINOMA CELLS; COMPLEMENTATION GROUP-1; ESCHERICHIA-COLI; GENE-EXPRESSION; MAMMALIAN-CELLS; RESISTANCE; LINES;
Keywords:
cisplatin; ovarian cancer; ERCC-1; platinum-DNA adduct; nucleotide excision repair;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Reed, E NCI, Med Ovarian Canc Serv, Med Branch,Dev Therapeut Dept, Div Clin Sci,NIH, Bldg 10,Room 12N226,9000 Rockville Pike, Bethesda, MD 20892 USA NCI Bldg 10,Room 12N226,9000 Rockville Pike Bethesda MD USA 20892
Citazione:
Q.D. Li et al., "Association between the level of ERCC-1 expression and the repair of cisplatin-induced DNA damage in human ovarian cancer cells", ANTICANC R, 20(2A), 2000, pp. 645-652

Abstract

Nucleotide excision repair (NER) is responsible for the repair of platinum-DNA lesions. ERCC-1 is a critical gene within the NER pathway, and cells without a functional ERCC-1 do not repair cisplatin-caused DNA damage. The present study was therefore designed to evaluate the relationship between the expression of ERCC-1 and the repair of cisplatin-induced DNA adducts in human ovarian cancer cells in vitro. One hour exposure of MCAS cells to cisplatin yielded an approximately two-fold increment in the levels of ERCC-1 mRNA and ERCC-1 protein, as determined respectively, by Northern and Westernblottings. In addition nuclear run-on assay showed that ERCC-1 gene transcription rate was increased to about the same extent as steady-state ERCC-1 mRNA and protein, in response to cisplatin treatment. However; the levels of ERCC-1 mRNA, ERCC-1 protein, and ERCC-1 transcript in MCAS cells are twofold lower. than those in A2780/CP70 cells, as previously reported. Furthermore, the repair of cispIatin-DNA adducts in MCAS cells, as measured,by atomic absorption spectrometry, is also nearly two-fold less than that in A2780/CP70 cells, indicating a strong association between the level of ERCC-1 expression and the activity of excision repair in these two human ovarian tumor cell lines. These results suggest that ERCC-1 may be a useful marker to monitor, the repair of platinum-DNA damage in tumor cells, and further highlight that potential pharmacological approaches which specifically inhibit ERCC-1 expression may increase cellular sensitivity to cisplatin.

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Documento generato il 25/06/18 alle ore 17:14:07