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Titolo:
Sequence, expression and functional analysis of the Coccidioides immitis ODC (ornithine decarboxylase) gene
Autore:
Guevara-Olvera, L; Hung, CY; Yu, JJ; Cole, GT;
Indirizzi:
Med Coll Ohio, Dept Microbiol & Immunol, Toledo, OH 43614 USA Med Coll Ohio Toledo OH USA 43614 crobiol & Immunol, Toledo, OH 43614 USA Inst Tecnol Celaya, Dept Ingn Bioquim, Celaya, Gto, Mexico Inst Tecnol Celaya Celaya Gto Mexico t Ingn Bioquim, Celaya, Gto, Mexico
Titolo Testata:
GENE
fascicolo: 1-2, volume: 242, anno: 2000,
pagine: 437 - 448
SICI:
0378-1119(20000125)242:1-2<437:SEAFAO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL REACTIVE PROTEIN; SACCHAROMYCES-CEREVISIAE; ESCHERICHIA-COLI; CANDIDA-ALBICANS; POLYAMINE; CLONING;
Keywords:
Coccidioides; complementation; gene function; genomic/cDNA sequences; ODC; protein expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Cole, GT Med Coll Ohio, Dept Microbiol & Immunol, 3055 Arlington Ave, Toledo, OH 43614 USA Med Coll Ohio 3055 Arlington Ave Toledo OH USA 43614 OH 43614 USA
Citazione:
L. Guevara-Olvera et al., "Sequence, expression and functional analysis of the Coccidioides immitis ODC (ornithine decarboxylase) gene", GENE, 242(1-2), 2000, pp. 437-448

Abstract

The ornithine decarboxylase (ODC) gene of the human respiratory fungal pathogen, Coccidioides immitis (Ci) was cloned, sequenced, chromosome-mapped, and expressed in Escherichia coli (Ec). The genomic, cDNA and translated sequences are presented. Transformation of an ODC null mutant strain of Ec (EWH 319) with the Ci ODC gene was conducted to confirm function of the protein encoded by the fungal gene. Activity of the enzyme by the bacterial transformant was inhibited by 1, 4-diamino-2-butanone (DAB), a known inhibitor of eukaryotic ODC. Temporal expression of the Ci ODC gene during the parasitic cell cycle is constitutive, based on results of RT-PCR. However, results of enzyme activity assays of cell homogenates obtained at different stages of parasitic cell development in vitro showed that the functional proteinis present only during periods of isotropic growth and segmentation, and these morphogenetic events can be arrested by the addition of DAB. The observed absence of a difference in steady-state mRNA transcript amounts, and the developmentally correlated Variation in levels of enzyme activity, suggest a translational or post-translational mechanism of ODC regulation. Since no PEST sequence was detected in the Ci ODC, enzyme regulation by programmed protein degradation as reported for many other eukaryotic ODCs may not occur in this case. ODC activity appears to play a key role in the morphogenesis of Ci, and the enzyme could be a rational target for therapy of disseminated coccidioidomycosis. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/06/18 alle ore 17:31:34